Yongjun Choi

Chief Technology Officer

About

Highly motivated biologist with several years of laboratory experience in molecular/cell biology, biochemistry and cell culture. Experienced in running research projects in collaboration with academic and industrial partners and designing experiments. Proficient in a wide array of techniques including recombinant DNA purification, microarray analysis, protein assay (PCR) preparation and validation, cloning, mammalian Cell Culture, antibody library prep/culture and basic microscopy

Country

South Korea

City

Cheongju

Industry

Research

Skill

Lentivirus production , Transduction CAR-T, Cytotoxicity assay, CAR expression measeured by FACS, Molecular Cloning, Protein Purification, Cell Culture, ELISA, Mouse Models, Protein-protein Interactions, Protein Production, Molecular & Cellular Biology, FACS analysis, Site directed mutagenesis, Fluorescence Microscopy, CRISPR-CAS9 guide RNA design and generation of point mutation Knock-in mouse, DNA-combing Assay, Cell survival assay induced by DNA damage agents, Confocal Microscopy, Mouse Xenograft

Experience

Chief Technology Officer

Cellengene Inc.

2022-3 - 2025-8 · 3 yrs 6 mos

Gyeonggi, South Korea

CAR-T cell therapy develop for solid tumors (Pancreatic cancer, ovarian cancer, mesothelioma, lung cancer)

CTO

AevisBio

2019-2 - 2022-2 · 3 yrs 1 mo

Yuseong-gu, Daejeon, Korea

Develop New Cancer drugs using PROTAC for Triple Negative Breast Cancer (TNBC). Develop Reposition drug for Neurodegenerative disease and Traumatic Brain Injury(TBI).

Production Manager

AniSciene

2018-1 - 2019-2 · 1 yr 2 mos

Jeonju, Jeollabuk-do, Korea

Proteins production using fermentor for animal food supplement.

Postdoctoral Research Fellow

Institute of Basic Science, Center for Genomic Integrity

2015-8 - 2017-12 · 2 yrs 5 mos

Ulju-gun, Ulsan, Korea

Work in Mouse embryonic section, Research topic is about hRAD51 mutations drived from human patients. Mouse embryonic stem cell expressing hRAD51 point mutations using its own promoter research the cellular phenotype; DNA damage reagents sensitivity, chromatin recruitment of hRAD51 with mouse endogenous RAD51, Replication fork protection and restart assay by DNA fiber assay.

Visiting Postdoc Fellow

The National Institutes of Health

2012-4 - 2015-7 · 3 yrs 4 mos

Bethesda, Maryland, United States

Investigation of function of small molecules for specific DNA repair pathway blocking. Mechanism study about PARP1 deficient cell sensitivity to small molecule. Antagonist study of ATAD5 induction