sharmistha acharya

Involved in designing expression-construct of immunological proteins, optimizing expression in mammalian, insect cell, E.coli system, purification through FPLC using GE Akta system and gravity columns as well as analytical evaluation of protein preparation that are headed towards crystal trial.

* Analyzed more than 100 coding and non-coding chromosomal regions of interests of mice provided by several investigators by performing high-throughput genotyping screening using molecular diagnostic techniques. * Performed data analysis and managed the analyzed data in the mouse database that was used in the variety of scientific experiments by different research groups of the institute. * Accomplished batch processing of more than 400 samples at one time using complicated protocols and delivered data to several clients by meeting their deadlines in a semi-industry setting.

* Managed a research laboratory of post doctorate scholars and graduate students by maintaining lab inventories, preparing solutions, organizing supplies and maintaining databases on a regular basis. * Contributed significantly in a xenograft based in vivo tumor modeling project with significant involvement in injections, monitoring, imaging and dissection of major organs in mice. * Developed and standardized Immuno-histochemistry protocol for tumor samples from mice to detect histo-pathological details following a post-xenograft viral therapy. * Prepared primary mouse fibroblast cell lines (MEF) and maintained them on a regular basis. * Prepared virus stocks using synthetic genomic techniques and tittered using ELISA assays.