Renato Bruni, PhD

Head: Eukaryotic Membrane Protein Expression @ New York Structural Biology Center

About

I am a Molecular Biologist with 20+ years experience in the field of molecular and cell biology with broad knowledge and skills in various aspects that emphasize membrane protein expression and purification, including cell-free systems, as well as biophysical characterization of proteins. I am seeking a Senior Research Scientist role because of my advanced technical, organizational and management skills. I have several years of supervisory, project management and cross-functional collaboration experience, and have trained and mentored scientists on topics specific to protein expression/purification, molecular cloning, and cell culturing. Additionally, I have strong analysis skills with the ability to implement and keep track of several projects simultaneously. Let's connect! I can also be reached by email at renatobruni66@gmail.com.

Country

United States

City

New York City Metropolitan Area

Industry

Research

Skill

Recombinant Protein , Molecular Cloning, Yeast two-hybrid, Protein Kinases, RNA Isolation, Cell Based Assays, GPCRs, insect cell expression, Baculovirus, Biophysics, Gel Filtration Chromatography, High Throughput, Laboratory Information Management System (LIMS), Membrane Proteins, Cross-team Collaboration, Project Management, Molecular Biology, Structural Biology, Cell Biology, Bioinformatics

Experience

Head: Eukaryotic Membrane Protein Expression

New York Structural Biology Center

2011-10 - 2023-3 · 11 yrs 6 mos

New York, NY

•Developed and maintained insect and mammalian cell expression platforms. •Improved workflow from target selection to expression and purification. •Collaborated with several outside groups on expression and purification of eukaryotic membrane proteins. •Implemented, optimized and published a cell-free expression method for membrane proteins. •Helped secure a NIH center grant in excess of $3,500,000.

Staff Scientist

New York Structural Biology Center

2007-5 - 2011-9 · 4 yrs 5 mos

New York, United States

•Engaged in devising a high-through put platform of bacterial membrane protein expression. •Contributed to the cloning, expression and purification of more than 10,000 membrane proteins that led to the 3D structure determination of more than 50 unique targets. •Published a step-by-step protocol for the high-through put cloning, expression and purification of bacterial membrane proteins. •Maintained and updated a proprietary laboratory information management system (LIMS).

Senior Research Scientist

CMT (acquired by Invitrogen, now Thermo Fisher)

2004-4 - 2007-3 · 3 yrs

•Led a group that cloned and tested 100 GPCRs using proprietary Tango technology, a cell-based functional assay. •Researched and developed different applications of the Tango technology for general use and for specific targets as requested by clients.

Senior Research Scientist

Tribeca

1998-10 - 2003-8 · 4 yrs 11 mos

•Designed and implemented strategies to identify several viral and cellular partners to the CMV UL97 Protein Kinase using two-hybrid system, immunoprecipitation, Protein Kinase assays and GST-fusion pull-down experiments. •Devised and constructed viral genomic library and mRNA library from virally infected cells.