Mareike Jaeckel

IVD Assay Development

@ Molecular Phytopathology group ▪ Design of fungal mutants and genetic analysis (transformation methods, Southern Blot), phenotypical analysis (morphology, biotests, analysis of virulence) and fluorescence microscopy of different fungal structures ▪ Analysis of fungal virus infection by infecting fungi with a dsRNA virus (eg. by purification and extraction of virus particles and subsequently transfection or Co-cultivation), analysis of virus infection on RNA level (eg. RNA Extraction, qRT-PCR) and protein level (e.g. ELISA, Western-Blot) ▪ Project planning, organization and execution ▪ Data analysis and preparation (MS Office, Origin, RStudio, Mega X) ▪ Conducting literature research and integrating findings into own research projects ▪ Knowledge in application and compliance with regulatory requirements of the German Genetic Engineering Act ▪ Supervision and grading of degree theses ▪ Teaching

Working Student / Master Thesis @ Molecular Plant Physiology group ▪ Recombinant production of proteins in E.coli including optimization of protein expression and solubility ▪ Protein purification via affinity and size exclusion chromatography followed by biochemical analysis (SDS-PAGE, Western Blot) ▪ Structure analysis of proteins (dynamic light scattering, CD spectroscopy, crystallization methods (vapor diffusion and batch crystallization)) ▪ Teaching and supervision of students

Internship @ Molecular Phytopathology group ▪ Classical cloning methods (restriction analysis, PCR, isolation of plasmid DNA, gel electrophoresis) ▪ Plant DNA / RNA isolation and purification ▪ Transformation methods in plants and bacteria

▪ Data analysis ▪ Enzyme activity determinations by photometric measurements (determination of stability (pH, temperature), determination of kinetic parameters) ▪ Gas chromatography and mass spectrometry

▪ Quality assurance ▪ Microbiological testing of food and drinking water ($64 LFGB-, DIN- and ISO-methods) ▪ Cultivation of microorganisms ▪ Evaluation of microorganism counting (MPN), detection and identification methods (catalase test, oxidase test, API tests, ELISA, microscopy)

▪ Doctor Thesis: Characterization of the proteins Hex-1 and SO in Fusarium graminearum and its influence on the vertical transmission of Fusarium graminearum virus china 9 (Magna cum Laude / 1.3) ▪ Institute of Plant Sciences and Microbiology (IPM) @ Molecular Phytopathology group

▪ Thesis: Investigation of the immune response in plants: structural analysis of a regulator protein (1.0) ▪ Labrotation 1: Expression and purification of the Histone H3 in A. thaliana (1.0) ▪ Labrotation 2: Recombinant production, purification and structural analysis of a membrane-bound plant protein (1.0)

Thesis: Biodistribution of two radioactively labelled iron oxide nanoparticles after intraperitoneal application in mice in long-term experiment (1.3)