lawrence hsieh

postdoctoral associate

About

A creative neuroscientist who takes an integrative approach to understand how genes regulate behavior through brain circuit development and how neuropsychiatric diseases alter normal brain circuitry. Using molecular biology and in utero electroporation to modify genes critical for normal brain development in vivo, I am able to elucidate their functional significance in shaping neuro-circuit. This approach paired with cell morphometry, electrophysiology, and behavior readouts enables me to fully understand how genes shape behavior.

Country

united states

City

new york

Industry

pharmaceuticals

Skill

immunohistochemistry, neuroscience, confocal microscopy, fluorescence microscopy, cell culture, western blotting, electrophysiology, drug discovery, cell, cell biology, rna isolation, transfection, hplc, enzyme assays, molecular cloning, animal models, pcr, immunoprecipitation, tissue culture, patch clamp, calcium imaging, expression cloning, signal transduction, heka, pclamp, in utero electroporation, acute brain slice preparation, 3 chamber sociability and social novelty test, marble burying task, fear potentiated startle, column chromatography, protein expression, protein purification, high performance liquid chromatography, optical microscopy, baculovirus, hesc/ipsc, gel electrophoresis, project delivery, scientific writing, pharmaceutical industry, neuropharmacology, compound screening, project management, biotechnology industry, cross functional team leadership, strategic planning, grantsmanship, creative solutions

Experience

research associate

genencor, a danisco division

2000-6 - 2001-9 · 1 yr 4 mos

* Privately Held * 1001-5000 employees * Biotechnology industry

graduate research assistant

uconn health

2005-9 - 2011-11 · 6 yrs 3 mos

* Educational Institution * 5001-10,000 employees * Hospital & Health Care industry

research associate 2

roche

2001-9 - 2005-7 · 3 yrs 11 mos

* Department of Central Nervous System (CNS) Diseases * Played an important role in the CNS disease drug discovery process by contributing intellectually during project meetings with medicinal chemists and group leaders, as well as purified heterologously expressed neuronal proteins to high throughput novel compound screen. * gt * To express proteins, Baculovirus and Sf9 cell suspensions were used. * gt * To purify proteins, HPLC with size-exclusion, ion exchange, affinity, and reversed-phase stationary phases were used. * gt * To characterize and validate proteins, SDS-PAGE, 2D-PAGE, western blot, spectrophotometry, circular dichroism spectroscopy were used. * Validated lead compounds using low throughput luciferase and radio ligand binding assays. * Successfully proposed summer internship projects and manage intern. * gt * Proposed project using novel photoproteins, Aequorin and Renilla, to develop new cell-free assay. * gt * Recruited intern from thousands of applications. * gt * Mentored intern -- from presentation of data to running HPLC.