Julie Silverman

Therapeutic Research Sciences, Cell Biology • Managed research activities of the Cell Biology team. • Developed flow cytometry cell-based assays to characterize binding and cellular trafficking dynamics of immune tolerance therapies (antibody-based and polymer conjugates). • Established an ex vivo antigen presentation assay using primary mouse T-cells to support mechanism of action activities. • Worked with CROs to generate antibodies for therapeutic candidates as well as reagents to support clinical assays. Collaborated with the bioanalytics and immunology teams to characterize and further optimize antibodies for downstream applications. • Managed cell-based IND-enabling activities and generated study reports for IND filings for KAN-101, an investigational treatment for Celiac Disease, now in Phase 1b/2.

• Designed and performed antibody affinity maturation campaigns on immune tolerance therapies using phage display technology. • Designed and generated protein expression constructs. Produced proteins using a mammalian cell-based protein production system to support in vivo proof of concept studies. • Generated stable CHO cell lines for cell-based assays.

Laboratory of Barbara Imperiali Research aim: Study the structural context of protein substrates of N-linked glycosylation in the human pathogen, Campylobacter jejuni, using biochemical and microbiological approaches. • Implemented an engineered E. coli system for expression of a non-native biosynthetic pathway • Performed kinetic studies on enzymes involved in N-linked glycosylation • Isolated and biochemically characterized a target protein-specific nanobody from a high-throughput phage-display screen • Led undergraduate research projects and provided mentorship • Provided expertise to team members for implementing bacterial culture and genetic techniques for Campylobacter jejuni

Laboratory of Joseph Mougous Research aim: Investigate posttranslational regulation and effector specificity of the type VI secretion system of Gram-negative bacteria. • Identified and characterized protein interactions that mediate substrate specificity of the type VI secretion system of bacteria • Described posttranslational regulators of the type VI secretion system and identified surface growth as an environmental signal • Collaborated with local and international research teams to integrate interdisciplinary techniques to study type VI secretion systems • Worked towards engineering protein nanotubes by DNA-templating using a type VI secretion system protein

Laboratory of David Davies Research aim: Examined the effects of small molecules on the dispersion of mutli-species biofilms.