Jane Hong

Proteins, Screening (HTS, DEL, ASMS, FBDD) Structural Biology, High Throughput Biophysics In Vitro Biology, In Vivo Pharmacology, Disease Models

• Research focuses on the role of ion channels in cardiovascular diseases and inflammatory disease • Designed in vivo cardiovascular testing and evaluated the effects of gene depletion on cardiac function in the cardiac disease model • Biochemical experimental design and performance, including primer design, DNA plasmid purification by maxiprep, RT-PCR, Western Blot, and immunohistochemistry • Protein interaction by immunoprecipitation (IP) • Cellular experimental design and performance on DNA plasmid transfection and adenovirus transduction, cell signaling assay • Evaluated the effect of small molecules on the ion channel activity in HEK293 cells and multiple primary cells by whole-cell patch-clamp recording • Presented work in groups and at academic seminars

• Research focuses on neuron-glia interaction and voltage-gated potassium ion channel in chronic heart failure and lung injury-induced cardiac arrhythmias; screened the expression of TRPV1 in multiple tissues by generating GFP transgenic mouse and confocal microscopy • Biochemical experimental design and performance, including tissue dissection, sectioning slices using a cryostat, immunofluorescence, ELISA, RT-PCR, and Western Blot • Make myocardial infarction mouse model of heart failure and acute ARDS, evaluated the inflammation by immunostaining, western blot, evaluate cardiac arrthymias and blood pressure by telemetry, evaluate cardiac function by echocardiography data analysis • Presented work in groups and at international academic conferences, wrote manuscripts • Experienced in summarizing hypothesises into graph abstract by Biorender

• Generated Parkinson’s mouse models and evaluated the neuroprotective effects of Sigma-1 receptor by measuring the neuron survival and death, inflammation, and behavioral phenotyping of the motor dysfunction • Evaluated the cognition and depression- and anxiety-like behaviors in Sigma-1 receptor • Evaluated the Impact of the low dose of BPA on male reproductive development by measuring the testis development and epigenetically regulation • Biochemical experimental design and performance, including genomic DNA extraction, genotyping, brain paraffin embedding, and sectioning, immunohistochemistry, tissue dissection, ELISA, RT-PCR, chromatin immunoprecipitation, and western blot • Analyzed data in EXCEL, Origin, and SPSS • Documented experiments and wrote manuscripts

Thesis title: The Effects of Bisphenol A on Embryonic Development and the underlying epigenetic mechanisms