Hong Ma

• Designed and constructed CRISPR screening for novel immunological targets • Validated target pathway using CRISPR technology and tool agents • Developed cancer target screening assays, such as protein-protein interactions

• Conducted in vitro cell based assays to screen for combos of drugs with synergy • Established resistant cell lines and screened with shRNA libraries to identify new targets • Worked with primary CLL patient cells for compound testing • Generated genetically-barcoded cell lines for in vivo models • Identified and established PD marker assays for immune cell activation • Worked with PDX tumor cells and supported screening with these models.

• Retroviral vector construction using the Gateway gene cloning system • Bone marrow and spleen cell isolation and flow cytometry analysis of markers

Designed shRNA oligoes and cloned them into shRNA vectors. Designed and cloned about 30 fusion protein constructs using bacterial and mammalian gene expression systems including adenoviral vectors.

Generated a knock-in mouse model for modulating islet function and survival post-transplant.

Supervised lab staff as a senior technologist in the lab Isolated primary rat myocytes Constructed adenoviral of wild-type and palmitoylation- defective mutant Beta2a-subunit of L-type calcium channel Designed and cloned four screening baits for screening in yeast two-hybrid system