Dominic Castanzo

Scientist II - Biochemistry and Characterization @ Metagenomi Therapeutics

About

Protein biochemist proficient in protein expression and purification, structural characterization, and assay development.

Country

United States

City

Emeryville

Industry

Biotechnology

Skill

E. coli, Crystallography, GraphPad Prism, Biochemistry, Protein Purification, Assay Development, Molecular Cloning, Molecular Biology, Fluorescence Microscopy, Confocal Microscopy, Polymerase Chain Reaction (PCR), SDS-PAGE, Protein Chemistry, Cell Culture, Column Chromatography, Immunoprecipitation, Mammalian Cell Culture, Molecular & Cellular Biology, Liposomes, X-ray crystallography

Experience

Scientist II - Biochemistry and Characterization

Metagenomi Therapeutics

2023-2 - Present · 3 yrs 8 mos

Emeryville, California, United States

Scientist I - Protein Science

Metagenomi Therapeutics

2021-11 - 2023-2 · 1 yr 4 mos

Emeryville, California, United States

Scientist - Protein/Antibody Engineering

Alamar Biosciences, Inc.

2020-8 - 2021-10 · 1 yr 3 mos

Hayward, California, United States

Graduate Student

University of California, Berkeley

2014-8 - 2020-7 · 6 yrs

Martin Lab, UC Berkeley

Department of Molecular & Cell Biology

Student Researcher

Ursinus College

2012-8 - 2014-5 · 1 yr 10 mos

Cameron Lab, Biology Department, Ursinus College

Assist in quantifying the endogenous rate of sup35 prion formation and loss in S. cerevisiae yeast. Performed confocal microscopy to evaluate aggregation of GFP-tagged Rnq1 in S. cerevisiae both with (wild-type) and without Zuo1 (∆zuo1).

Student Researcher

Ursinus College

2011-1 - 2014-5 · 3 yrs 5 mos

Lyczak Lab, Biology Department, Ursinus College

Used confocal microscopy to study the centrosome's role in the establishment of the anteroposterior axis in C. elegans embryos. Crossed various C. elegans strains to produce a lineage with multiple GFP-tagged proteins to track the migration of PAR-6, y-tubulin, and Histone H2B throughout the first division of the C. elegans zygote.

Summer Research Student

The Ohio State University

2013-6 - 2013-8 · 3 mos

Fisk Lab, Department of Molecular Genetics

I worked under Dr. Harold Fisk the study the tole of STS1 and ZAP70 in the degradation of the Mps1 kinase, which plays a role in centrosome duplication. I cultured mammalian cells (HEK293, HeLa, and Rpe1 cells) and performed fluorescence microscopy to monitor centrosome duplication in cell lines hosting either wild-type or mutant Mps1 kinase. Performed immunoprecipitations from cell lysate to determine interaction between GFP-tagged STS1 and Mps1.