Zichen Qian

• Ph.D with 8 years’ combined academia and industrial experience in cell engineering, tissue engineering, cell line development, drug discovery and development • Experienced in stable cell line development and biologics production • Experienced in fast-paced R&D laboratory environment, familiarity with GLP guideline • Excellent academic performance presented by 14 peer-reviewed papers and 4 research awards • Experience with grant writing. Great verbal and writing communication skills presented by oral and poster presentation in national conferences • Mentored 4 junior Ph.D students, 1 Master students and 1 undergraduate students Skills and Technique: • High-throughput assay: Single cell sorting and cloning (Aria, Cellavista), single clone screening and immunological assay (Tecan, Guava, Octet), automated fed-batch production and stability studies (Ambr) • Cell culture: mammalian cell culture (CHO, Stem cell, PBMC, Neuron), primary cell (PBMCs, T cells, neuron) isolation, 3D cell culture, cell banking and bioreactor cell culture • Molecular biology skills: transfection (Neon, Nucleofector), immunofluorescent staining, western blot, RT-qPCR, flow cytometry, zymography, ELISA, MTT/XTT, BCA, Octet, in situ ELISA • Fabrication: Decellularization of cell sheet, isolation of ECM, photolithography, patterning of cell culture surface, Solid Freeform fabrication • Animal model: Rat abdominal aorta xenografting, mouse subcutaneous study, animal handling and maintenance • Software skills: Microsoft Office proficiency, MATLAB programming, ImageJ, JMP statistics, Sigma plot, Adobe photoshop, Adobe illustrator, ELN documentation