Yohei Ohashi
Investigator Scientist @ Medical Research Council
About
I am a multidisciplinary researcher with over 20 years of professional experience in various biology subjects, covering from molecular/cell biology to structural biology. I am currently working on Class III PI3K complexes (VPS34 complexes) using cell biological, biochemical, biophysical, and structural biological methods in the laboratory of Roger Williams, FRS at the MRC-LMB, Cambridge, UK. I usually work as a team member, whereas I can also work independently when necessary. I am also on the Green Committee at the MRC-LMB, where I am keen on laboratory sustainability. Main accomplishments: -Developed an assay method to quantitatively measure lipid kinase activities (Ohashi et al., eLife, 2020) -Developed protein expression and landmarking methods for cryo-EM using a thermotolerant yeast, K. marxianus (Baretić et al., Nat Commun, 2016) -Developed a multiple-protein expression system using S. cerevisiae (Rostislavleva et al., 2015, Science) -Characterised membrane traffic pathways in autophagy (Ohashi and Munro, MBC, 2010) -Developed transgenic plants that can inducibly generate root hairs at any organs (Ohashi et al., Science, 2003) Main skills: -Problem solving -Supervision -4000+ DNA constructs for cell biology, structural biology, and transgenic organisms etc.. -Developing protein expression systems using eukaryotic cells (mainly yeast and mammalian cells) -Protein purification optimisation for large protein complexes with multiple subunits ( ~ 1.5 MDa) -Nanobody characterisation (screening, engineering etc...) -Cell biology skills -Molecular biology skills -Biochemistry skills -Yeast and plant genetics Other profiles: -ORCID: https://orcid.org/my-orcid?orcid=0000-0002-2288-130X -Research Gate: https://www.researchgate.net/profile/Yohei-Ohashi-3 -Loop: https://loop.frontiersin.org/people/319669/overview
United Kingdom
Greater Cambridge Area
Biotechnology
Protein Expression, Protein Purification, Biochemistry, Yeast, Molecular Biology, Fluorescence Microscopy, Cell Biology, Membrane Trafficking, Scanning Electron Microscopy, Autophagy, Confocal Microscopy, Protein-protein Interactions, Transcriptional Regulation, Genetics, Botany, Liposomes, Enzyme Kinetics, Supervisory Skills, communication, Biotechnology
Experience
Investigator Scientist
Medical Research Council
イギリス ケンブリッジ
Laboratory of Roger Williams -Developed a method to quantitatively measure lipid kinase activities (Ohashi et al., 2020, eLife) -Examined the importance of somatic mutations and post-translational modifications in human VPS34 complexes (Ohashi et al., 2018, JLR) -Characterised the fifth subunit of ClassIII PI3K complex I, Atg38 (yeast) and NRBF2 (human) structually, biochemically, biophysically, and cell biologically (Ohashi et al., 2016, Autophagy).
Career Development Fellow
Medical Research Council
MRC Laboratory of Molecular Biology
Laboratory of Roger Williams -Developed multiple protein expression systems using S. cerevisiae for studying Class III PI3Ks, along with protein purification optimisation and cell biology (Rostislavleva et al., 2015, Science) -Developed a new protein expression system using a thermotolerant yeast, K. marxianus. Also developed an electron density marking method using tandem-RFP for cryo-electron microscopy (Baretić et al., 2016, Nature Comm).
Post-doctoral researcher
Medical Research Council
イギリス ケンブリッジ
Laboratory of Sean Munro (funded by EMBO, HFSP, JSPS, and MRC) -Initiated yeast autophagy study -Found that a membrane source of the autophagosome is the Golgi (Ohashi and Munro, 2010, MBC)
Associate Instructor
Kyoto University
Kyoto, Japan
-Involved in teaching Ph.D students -Worked on functional analysis of a homeobox protein and a cyclin protein using molecular biological, cell biological, and bioinformatical methods (Imai et al., 2006, Plant Cell).
Post-doctoral Research Fellow
Bio-oriented Technology Research Advancement Instruction
Kyoto University
-Designed and generated transgenic plants harboring ectopic root hairs upon dexamethasone induction. Using these I elucidated the involvement of a phospholipid signaling pathway in cell morphogenesis using molecular biological and cell biological approaches (e.g., Ohashi et al., 2003, Science)
Education
Kyoto University
Molecular Biology
-Molecular Biological and physiological analysis of a transcription factor, GL2 using transgenic plants (Imajuku et al., 2001; Ohashi et al., 2002, Plant J) Supervisor: Takashi Aoyama
Kobe University
Biology, General
Undergraduate study (1996-1997) Phycological study on an order of the brown algae, Dictyotales. -Classified the species based on the results of intracellular PH measurements, DNA sequencing,and field work. Supervisor: Hiroshi Kawai
Yohei Ohashi's Contact Information
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