Yi-ying Chou

- Established CRISPR based functional genomic screening capacity at Biogen in both arrayed (high throughput screening, HTS) and pooled formats; for both CRISPR knockout and CRISPR activation screenings. - Lead multiple cross-functional CRISPR screening projects for small molecule drug target identification, disease biology discovery and improvement of various therapeutic modalities: antisense oligonucleotides (ASO), AAV gene therapy - Pioneered in the quality control method for arrayed CRISPR screening - Manage and mentor an associate scientist - Established a CRISPR strategy group to coordinate efforts and research tools across the drug discovery organization - Facilitated application of induced pluripotent stem cell (iPSC)-based assays for CRISPR screening projects - Generate and validate engineered cell lines to support assay development for compound target validation, high throughput screens, and pathway analysis.

- Pioneering and establishing arrayed CRISPR screening platform for small molecule drug target identification, cellular pathway discoveries - Lead CRISPR screening projects involving various therapeutics modalities: AAV gene therapy, ASO and small molecule drug - Manage and mentor an associate scientist

Undergraduate course: Genetics, Genomics and Evolution course (LS1b) Class size: about 300 students Responsibility: Teach a weekly 3-hour section including course material discussion and lab activities. I also grade weekly problem sets and lab assignments, hold office hours to help students learn the course material.

Harvard Medical School, Boston MA Lab of Dr. Tom Kirchhausen Lab focused on using high-resolution live-cell imaging to study cellular membrane and virus trafficking Post-doctoral researcher • Discover and demonstrate the mechanisms of small molecule inhibitor against Junin virus. • Research focused on developing imaging system to study JC polyomavirus entry and penetration in infected cells. Used lattice-light sheet microscopy, spinning disc confocal microscopy and CRISPR/Cas9 gene editing technology. • Manage and establish the molecular cloning and CRISRP/Cas9 gene editing workflow in the lab and help generated more than 20 gene edited cell lines. • Used molecular biology, cell biology, tissue culture, immunofluorescence, live-cell imaging extensively. • Participating in collaboration with multiple research labs including the department of microbiology at Harvard, Biogen and others. • Trained and supervised technicians in the management of gene editing core in the lab.

Icahn School of Medicine at Mount Sinai, New York City, NY Lab of Dr. Peter Palese. The Lab focused on biology of influenza viruses and the development of universal influenza vaccine Doctoral researcher • Developed single-molecule sensitivity fluorescence in situ hybridization (sm-FISH) technique for visualization of influenza viral RNAs. • Provided evidences showing that influenza virus packages its genome in a selective manner and elucidate the packaging process in infected cells. • Identified M segment as a critical gene for the transmissibility of 2009 pandemic influenza virus in the Guinea pig model. • Utilized transmission electron microscopy, cryo-EM, negative staining to reveal the morphology of influenza viruses, influenza virus like particles during vaccine production and cellular proteins. • Practiced virology techniques extensively: amplification of influenza virus in chicken embryos, reverse genetics of influenza virus, plaque assay, fluorescent microscopy. • Collaborated with various labs and research institutes including: University of illinois at Urbana-Champaign, Albert Einstein College of Medicine.

Graduate course: Cell biology and signal transduction. Class size: 35 students Designed problem sets, graded written homework, gave lectures at problem set sessions, moderated student journal clubs and met with students upon request

Major in Life Science Undergraduate research in Dr. Shau-Chi Chi's lab • Defined the role of autophagy in Nervous Necrosis Virus infection in grouper fin cells.