Xiuling Liu

> Led research collaboration and customized product development of high brightness fluorescent probes for mRNA targeting, resulting in two filed patents. • Created and functionalized nanodots with reactive linkers by radical reactions. • Designed optimal sequences of oligonucleotides as primary probe for selective mRNA targeting. • Performed bioconjugation, purification and characterization by HPLC, SEC, TBE-Gel, Fluorescence, Uv-vis, FTIR, SEM, AFM and TEM. • Validated the products through complementary hybridization captured by streptavidin beads and glass slides and applied the probes to detect beta-mRNA in Hela cells by Fluorescence in situ Hybridization (FISH) techniques. • Skilled in organic reactions, cell culture, fixation, optimization of hybridization and washing protocol and fluorescence microscopy (Confocal, Widefield and TIRF/HILO). > Wrote and won NSF STTR Phase I Award as Principal Investigator and completed NSF I-Corp workshop on technology transfer, customer discovery and finding the pilot of the technology.

* Developed antibody-conjugated high brightness probes for antigen CD4 and CD19 detection in PBMC cells through flow cytometry. • Performed process development to create stable formulation for nanobutes. • Introduced linkers and functional groups to nanotubes to produce high-brightness fluorophores and probes. • Optimized chemical modification of antibody and bio-conjugation reaction efficiency and separated and characterized the products through chromatography, SDS-PAGE, UV-vis, and ELISA. • Validated the final product by demonstrating high brightness of the final product with flow cytometry. • Published one review paper and filed one patent on covalent functionalization of high-brightness fluorophores.

> Developed adenosine-functionalized biodegradable polymers-based nanoparticles for treatment of Osteoarthritis. • Designed and synthesized six adenosine-terminated PLA-PEG block copolymers with different modification positions and formulated them into nanoparticles. • Studied the relationship of functionalization site and biological activity, discovered the key modification position. • Performed chemical modification and nanoparticles synthesis, purified and characterized products by NMR, DLS, TEM, and UV-vis. • Demonstrated the efficacy of the nanoparticles on treatment of Osteoarthritis in a mouse model.

Synthesis and Chiroptical property study of Copper(I/II) complexes.

1.Tutored and guided undergraduates on Organic Chemistry Lab 2. led weekly discussions and office hours.

1. Developed several fluorescent sensors for detection of biologically and medically relevant molecules including H2S, thiolphenols and fluoride ion. 2. Studied competitive mechanism between Paternò-Büchi reaction and triplet-triplet energy transfer reaction. 3. Designed and synthesized Quinoline and Bodipy derivatives for dye-sensitized solar cells 4. Managed lab instruments such as HPLC, Electrochemical work instrument, UV/vis spectrometer and fluorescence spectrometer and so on.