Xinyi Wu

Proteomics: Post-translational modification analysis (phosphorylation, ubiquitination). Untargeted quantitative proteomics using label-free (LFQ) or isotope labeling techniques (SILAC). Targeted protein quantitation using Parallel-Reaction Monitoring (PRM). Protein characterization (amino acid sequencing, intact mass analysis) using bottom-up and top-down LC-MS/MS. Drug-protein adduct analysis using LC-MS/MS based proteomics. AP-MS analysis for the identification of protein-protein interactions. Protein-protein interaction and kinetics detection using biosensor technologies. Metabolomics: In-depth expertise of sample preparation and extraction from complex matrices. Targeted metabolite identification/quantitation using LC-MS/MS. Untargeted metabolomics analysis and differential screening of changed metabolites in transfected cell lines.

Demonstrated skills and high efficiency in peptide mapping, protein/protein interaction, protein characterization, protein-drug conjugate analysis, PTM analysis, quantitative analysis in single protein or biological matrices using bottom-up and top-down LC-MS; performed associated data interpretation • Developed nano-flow and micro-flow LC-MS system for metabolite quantitation, identification; aided in metabolite spectrum database setting up and search engine establishment • Highly experienced in separation techniques of proteins and peptides (affinity enrichment, SPE/LLE) interfaced with mass spectrometry (micro/nano LC) • Introduced PRM method for LC-MS system quality control • Familiar with Python to handle high-content data • Extensive hand-on experiences and trouble-shooting proficiency in method development, instrument maintenance and troubleshooting for the instruments including MALDI TOF, LTQ Orbitrap, Q Exactive, Easy-nLC 1000, Agilent 1100 HPLC and Eksigent HPLC • Collaborated in a multidisciplinary research environment and delivered results in line with project objectives frequently • Successfully addressed bioanalytical issues related to discovery programs

• Effectively developed primary endothelial cell (EC) isolation method from mouse liver and established the culture condition; built an efficient way for IFNγ Receptor staining method using flow cytometry/RT-PCR and employed it for the genotyping work • Applied a serial of techniques including immunocytochemistry/immunofluorescence, cytometric bead array, western blot and illustrated the mechanism of IFNγ on ECs in tumor angiostasis • Mastered a very difficult intrahepatic lymphocytes isolation procedure and successfully applied it to the determination of the frequencies of Th17 cells and its apoptotic status in total liver lymphocytes • Performed complicated Th17 induction in vitro experiment and intracellular staining method; demonstrated the important role of IFNγ in keeping Th1/Th17 balance during virus infection • Conducted a comprehensive study to map a endogenous TNF signaling and demonstrated TNF maintains myeloid-derived suppressor cells (MDSC) survival and promotes tumor growth

Produced a monoclonal antibody against human IgG in Balb/c mouse