Xichan Hu

Senior Director @ Aureka Biotechnologies, Inc.

About

• 16 years research experience in Biochemistry, Molecular Biology, Cell biology. Extensive experience in protein biochemistry, NGS/RNA biology, cancer biology/epigenetic and lipid metabolism.  • 7 years experience in protein expression/purification from E.coli, yeast, insect and mammalian cells, protein characterization and protein-protein interactions.  • 14 years experience in cell culture, stable cell line generation, cell based essays and mouse model. • 7 years experience in human lipoproteins separation, cholesterol/oxysterol delivery, cell/lipoprotein cholesterol/oxysterol extraction and determination.  • 7 years experience in RNA transcription&purification, RNA secondary structure determination, NGS library preparation, RNA/DNA-protein binding interfaces. • 7 years experience in radioactive labeling related experiments, such as EMSA, SHAPE, foot printing and enzyme activity essays. • 3 years experience in sectioning and immunohistochemistry.

Country

United States

City

Irvine

Industry

Biotechnology

Skill

NGS, Protein expression/purification/engineering, Yeast/insect/mammalian/stem cell culturing, cell based assays, Southern/western/northern blot, SHAPE, SHAPE-seq, Foot printing, mouse model/xenograft model, Primary hepatocyte and PBMC separation and culturing, Human plasma lipoproteins separation, ITC, Octet, Co-IP, ChIP, EMSA, Immunofluorescence/immunocytochemistry/immunohistochemistry

Experience

Senior Director

Aureka Biotechnologies, Inc.

2026-3 - Present · 7 mos

United States

Director

Aureka Biotechnologies, Inc.

2023-7 - 2026-3 · 2 yrs 9 mos

Irvine

Senior Scientist

Chantibody Therapeutics Inc.

2022-6 - 2023-7 · 1 yr 2 mos

United States

Senior Scientist

Henlius USA Inc.

2021-7 - 2022-6 · 1 yr

Milpitas, California, United States

Protein purification and analytics

Associate Specialist

UC Irvine

2020-7 - 2021-7 · 1 yr 1 mo

Irvine

-Design and optimize foot printing, ITC, Octet and EMSA experiment, discover the special binding mode between hLARP7 and RNA. -Develop and perform protein expression in insect cells using recombinant baculovirus.

Postdoctoral scholar

UC Irvine

2015-7 - 2020-6 · 5 yrs

Irvine, California, United States

-Design and optimize Co-IP, ChIP, cloning, FPLC & ion exchange, GST/streptavidin pull-down, ITC, Octet, southern blot, northern blot to validate the hypothesis generated from LC-MS and cross-link LC-MS, discover TEL-patch dependent telomerase recruitment in yeast. -Solve the RNA secondary structures by establishing SHAPE, SHAPE-seq, exploring and implementing computational methods, discover the quality control of telomerase RNA. -Purify and validate antibodies for telomere-associated proteins. -Design and perform immunofluorescence to screen the compounds in human U2OS/HepG2/PC9 cells for killing tumor cells through targeting telomere-associated proteins. -Formulate hypothesis, write manuscripts for publications. Effectively communicate scientific findings on a regular basis in meetings and symposiums to scientific collaborators and non-specialist.

Junior Specialist

UC Irvine

2014-4 - 2015-6 · 1 yr 3 mos

Irvine, California, United States

-Develop and perform overexpression, cell cycle synchronization, Co-IP and ubiquitin pull-down assy, discover cell cycle dependent degradation of telomere-associated proteins responsible for telomere maintenance.

Doctoral Student

University of Chinese Academy of Sciences

2009-9 - 2014-1 · 4 yrs 5 mos

Shanghai, China

-Establish method for low abundant lipid (oxysterols) detection by GC-MS, perform cell growth assay and xenograft, and discover induced ACAT2 as a biomarker of hepatocellular carcinoma (HCC) and target for treatment of HCC. -Develop a system to screen specific enzyme (ACAT2) inhibitors in CHO cells using NBD-sterol.

Research Associate

University of Chinese Academy of Sciences

2007-9 - 2009-8 · 2 yrs

Shanghai, China

-Design and perform bisulfite genomic DNA sequencing, luciferase activity assay, ChIP and gene knockdown, discover C/EBP transcription factor responsible for ACAT2 gene expression in human THP-1 cell.

Masters Student

Northeast Normal University

2004-9 - 2007-7 · 2 yrs 11 mos

Changchun, China

-Discover specific tryptophan hydroxylase and map serotonin signal pathway in the brain of the beetles by using neuroanatomy, sectioning and immunohistochemistry techniques.