Xiaojing Wang

• Developed and validated stability-indicating HPLC/UPLC methods for assay, related substances, and residuals in accordance with ICH Q2 guidelines • Solid-state characterization; Polymorph screening and Crystallization • API Grinding and Sieving, Particle size distribution (PSD) method development • Dissolution study of API in biorelevant media (FaSSGF, pH 1.6; FaSSIF, pH 6.5; FeSSIF, pH 5.0) • Designed and executed forced degradation studies to establish degradation pathways and demonstrate method specificity • Conducted drug product stability studies and trended data to support shelf-life assignment and specification setting • Supported residual solvent testing (GC), moisture analysis (Karl Fischer), and thermal characterization (TGA/DSC) to assess critical material attributes impacting product performance • Established in-process controls (IPC) and provided analytical support for manufacturing scale-up and process optimization • Authored analytical protocols, validation reports, technical documents, and contributed to CMC sections for regulatory submissions • Collaborated cross-functionally with formulation, process development, QA, and regulatory teams to ensure successful technology transfer to QC

• Developed and validated a lead assay based on DNAzyme and blood glucose meter (BGM) detection • Conjugated and purified oligonucleotides and proteins, immobilized DNA-invertase on magnetic beads

Co-mentored by Prof. Yi Lu and Prof. Brian T. Cunningham • Led the develop an ultrasensitive exosomal miRNA detection assay, enabling early cancer diagnosis • Develop state-of-the-art analytical methods for the release and characterization of oligonucleotide-based sequences; qRT-PCR assay optimization and validation • Conducted and purified the Antibody Oligonucleotide Conjugates (AOC) to construct a polyvalent nanobody drug for efficient inhibition of the SARS-Cov-2 virus at lower dosage levels • Fabricated and characterized a lateral flow assay based on the interaction between oligonucleotide aptamers and SARS-CoV-2 • Fabricated lipid nanoparticle (LNP) and encapsulate nucleic acid, characterization, and purification • Developed a remotely controlled DNAzyme-based detection method using ultrasound, which achieved non-invasive and spatiotemporal metal ions imaging in both cancer cells and mouse models • Assisted the Principal Investigator in grant and patent application, including literature survey, data analysis, and proposal drafting • Provided training to graduate students in experimental design, data collection, and publication

Mentored by Haojun Liang • Designed and developed delivery strategies for our nucleic acid-based modality, and related liposomes nanoparticle characterization • Designed a signal-amplified DNA machine for sensing cancer biomarkers in vivo conditions that established a sensitive fluorescent miRNA imaging strategy and imaging-guided photothermal therapy • Acquired funding and served as leader of Youth Fund Project of National Natural Science Foundation of China 2016 with research focused on “Monitoring the dysexpression of microRNA in cancer cell based on DNA molecular machine transfering with peptide nanoparticles” • Established and managed a cell research team in the laboratory by training graduate students in cell culture techniques, transfection methods, confocal imaging, and flow cytometry

• Synthesized and purified peptide-drug conjugates (PDC) to overcome multidrug resistance, HPLC, ESI-MS, NMR analysis of peptide and small molecule’s structure characterization and elucidation • Designed biocompatible peptides with small molecules for imaging cellular metal ions and proteins • Probed the mechanism of the Pd(II)-catalyzed decarboxylative reaction, capturing organic reactive intermediates using ESI-MS/MS • Identified small molecules (e.g., L-carnitine, omeprazole) in biological matrices with LC-MS/MS, provided bioanalytical support for in-vitro DMPK studies • Conducted mass spectrometry analysis (i.e. LC-MS, ESI-MS/MS analysis) on 2000+ samples and provided technical consultation for service users • Mentored students in the peptide synthesis (SPPS), small molecular modification, peptide-based drugs bioconjugation and purification, and trained them on the operation of Agilent/Shimadzu/Waters HPLC-UV and Thermo Fisher Scientific LC-MS/MS, ESI-MS/MS • Prepared lectures and class activities focusing on Organic Chemistry for 40-60 undergraduates;

• Prepared lectures and class activities focusing on Organic Chemistry for 40-60 undergraduates • Created and graded course assessments, and held office hours to ensure students understood class materials and stayed on track

• Synthesized and purified peptide-drug conjugates (PDC) to overcome multidrug resistance, HPLC, ESI-MS, NMR analysis of peptide and small molecule’s structure characterization and elucidation • Designed biocompatible peptides with small molecules for imaging cellular metal ions and proteins • Probed the mechanism of the Pd(II)-catalyzed decarboxylative reaction, capturing organic reactive intermediates using ESI-MS/MS • Identified small molecules (e.g., L-carnitine, omeprazole) in biological matrices with LC-MS/MS, provided bioanalytical support for in-vitro DMPK studies • Conducted mass spectrometry analysis (i.e. LC-MS, ESI-MS/MS analysis) on 2000+ samples and provided technical consultation for service users • Mentored students in the peptide synthesis (SPPS), small molecular modification, peptide-based drugs bioconjugation and purification, and trained them on the operation of Agilent/Shimadzu/Waters HPLC-UV and Thermo Fisher Scientific LC-MS/MS, ESI-MS/MS