Xiaoqing Hua

Manage a team of scientists working on early and late stage biologics pipeline. Drive analytical control strategy for new modalities.

• Support in-process intermediates characterization of mAbs, including sample impurities test with UPSEC assay, charge variants estimation using IEX assay, and oxidation impurities testing with HIC method • Developed and pre-qualified a cIEF assay to estimate charge variants of bi-specific antibody • Use 2D-DIGE and 2D-Gel combining with Western blot to determine HCP coverage of poly-clonal antibody

• Developed a high-throughput sample preparation method for N-Glycan profile analysis using UPLC/mass spec • Established a high-throughput Biacore-based affinity assay to quantify bi-specific/multi-specific antibodies • Worked in a matrix environment and provided in-process analytical support to clone selection and process development

• Established a SEC-HPLC method for in-process sample testing with LOQ as low as 50ng/mL • Developed and optimized an UPLC method to quantify amino acids concentrations with high sensitivity (1 uM on column) and accuracy (<10% error) to support internal CHO cell culture media development • Established a capillary electrophoresis (CE-SDS) method to examine the purity and structure of purified antibodies

• Quantitatively measured particle-particle interactions at liquid-liquid and solid-liquid interfaces, as well as particle-surface interactions under different solvent conditions to better understand and tune the experimental system • Synthesized and characterized gold nanoparticles (NPs) with novel surface modifications and reversible adsorption properties, which can be widely applied to thermodynamically stabilized Pickering emulsions, surface plasmonics, and catalysis of bi-phase reactions • Measured and verified the Gibbs adsorption isotherm of NPs at fluid-fluid interface via UV-Vis spectroscopy • Resolved one of the most fundamental and controversial theories in NP-coated interface by demonstrating that interfacial tension reduction is due to the removal of interfacial area by NPs • Modeled particle adsorption dynamics which provides essential binding characteristics at fluid-fluid interface

Focused on statistics and data analysis, experiences with statistics tool such as R, Python and MATLAB.

• Performed solid phase synthesis for a thermal sensitive elastin-like polypeptides (ELPs), characterized its sequence and structures, and accurately determined the critical micelle concentration (CMC) with interfacial tension measurement • Characterized the thermo-sensitive interactions between ELPs-formed micelles and hydrophilic surfaces • Explored a novel way to tune silica colloids assembly via temperature-controlled ELP micelle structure formation