Wenyan Xu

Develop, validate, and execute GMP-compliant analytical methods for pharmaceutical and biopharmaceutical samples using advanced chromatography, electrophoresis, and compendial techniques. Proficient in aseptic technique and handling complex biologics, including monoclonal antibodies, recombinant proteins, antibody–drug conjugates, and vaccines. Responsible for implementing, maintaining, calibrating, and operating analytical instrumentation, and for documenting all activities in alignment with GMP and GxP requirements. Perform work according to SOPs with strong attention to detail, accuracy, and organization. Collaborate effectively within cross-functional teams, serving as a technical lead and mentor for junior staff while supporting a high-quality, compliance-driven laboratory environment.

I provide professional scientific consulting and AI-trainings. My clients include Outlier, Amazon, Semantix, ClearVoice, etc.

I lead a team of ten dedicated professionals, overseeing all aspects of performance, safety, and employee relations. This includes responsibilities such as interviewing, hiring, and performance management. Together with my exceptional team, we consistently deliver outstanding scientific services, ensuring client satisfaction through our in-sourcing model.

I collaborate with a premier Biopharma client, delivering specialized scientific services customized to individual requirements. My expertise spans: Purification of Small Compounds: Employing cutting-edge techniques to isolate and refine small molecules, ensuring purity and efficacy. Development and Validation of Bio-analytical Methods: Spearheading innovative approaches to establish and verify bio-analytical methodologies, enhancing accuracy and reliability. Process Automation: Driving operational efficiency by implementing advanced automation solutions, streamlining workflows and maximizing productivity.

I collaborate with a premier Biopharma client, delivering specialized scientific services customized to individual requirements. My expertise spans: Purification of Small Compounds: Employing cutting-edge techniques to isolate and refine small molecules, ensuring purity and efficacy. Development and Validation of Bio-analytical Methods: Spearheading innovative approaches to establish and verify bio-analytical methodologies, enhancing accuracy and reliability. Process Automation: Driving operational efficiency by implementing advanced automation solutions, streamlining workflows and maximizing productivity.

I supported Dr. Linlin Zhao's research as a senior scientist. My duties included: Method Development: Developed an ELISA-based method for detecting DNA-protein crosslinks in biological samples. This method was applied in numerous research projects, resulting in one published discovery. Contributed to the development of an LC-MS/MS-based method for the detection and quantification of single nucleoside adducts. Lab Management: Ensured all laboratory processes complied with Good Laboratory Practice (GLP) guidelines. Duties included writing safety protocols, such as those for biosafety and waste disposal, for committee review at the university level. Cell Culture and Treatment: Maintained mammalian cell lines, administered treatments, and investigated cellular responses. Training and Supervision: Trained and supervised students in protein overexpression and purification protocols I developed. Designed site-directed mutagenesis for residue substitutions and constructed plasmids for protein overexpression when new protein variants were required.

I supported Dr Linlin Zhao's research as a senior scientist. The duties included: Method Development: Developed an ELISA-based method for detecting DNA-protein crosslinks in biological samples. Applied in numerous research projects, resulting in one published discovery. Contributed to the development of an LC-MS/MS-based method for the detection and quantification of single nucleoside adducts. SOP Preparation and Lab Management: Prepared Standard Operating Procedures (SOPs) and managed the laboratory to ensure compliance with Good Laboratory Practice (GLP) guidelines. Cell Culture and Treatment: Maintained mammalian cell lines, admitted treatments, and investigated cellular responses. Training and Supervision: Trained and supervised students in protein overexpression and purification protocols developed by me. Designed site-directed mutagenesis for residue substitutions and construct plasmids for protein overexpression when new protein variants are required.

I developed protocols for the overexpression and purification of proteins. The proteins include DPO1, DPO4, human PrimPol, human TFAM, human DNA polymerases iota and eta, human RPA, and yeast RPA. The discoveries have been published in several journal papers. I was selected as awardees in several academic conferences such as the American Chemical Society national conferences and the annual serial conference of the United Mitochondrial Disease Foundation.

I developed protocols for the overexpression and purification of proteins. The proteins include DPO1, DPO4, human PrimPol, human TFAM, human DNA polymerases iota and eta, human RPA, and yeast RPA. The discoveries have been published in several journal papers. I was selected as awardees in several academic conferences such as the American Chemical Society national conferences and the annual serial conference of the United Mitochondrial Disease Foundation.

I worked on the self-assembly of plant virus, which could lead to construct new functional bio-nanomaterials. With Fluorescence labeled cyclotide, we have confirmed that the aggregation of MCTI-I or MCoTI-II at w/o interface by Co-focal Laser Scanning Microscopy (CLSM). The thickness of dried capsule of cyclotides investigated by Atom Force Microscopy (AFM) indicates that the molecules form membrane in single molecule layer at the liquid interface. We have not observed any ordered structures in nano-scale for MCTI-I either in the membrane or in the solution by Small Angle X-ray Scattering (SAXS) and X-ray Diffraction (XRD), and we are investigating the structure constructed from another cyclotide MCoTI-II.

1. Biosynthetic Pathway of Heterophyllin B (HB). (This is a collaborative project of Prof Tan and Prof Du) The amounts of HB in Pseudostellaria heterophylla (Caryophyllaceae) at different growing stages and in different materials cultured were analyzed. And further, culture systems (including shoots and hairy roots) were established for the research on biosynthetic pathway of HB either in vitro or in vivo. 2. Mechanisms of Fumonisins Resistance The content of lipids and fumonisins in the wild type strain as well as in the individual FUM mutants was analyzed by high performance liquid chromatography and MS. The results suggest that FUM17 and FUM18 are ceramide synthase and that the functions of these FUM genes are probably redundant due to the presence of other resistance genes in the Fusarium verticillioides genome. Consequently, efforts to delete an additional gene, FUM11, were carried out.