Wei Jie Wong

•Designed, generated, and characterized novel CAR constructs supporting next-generation cell therapy programs •Optimized upstream and downstream CAR-T manufacturing processes including T-cell activation, gene modification, expansion and harvest •Established and executed QC assays for identity, purity, potency, vector titration, and product characterization •Performed multiparameter flow cytometry for immunophenotyping, transduction efficiency, and in-process analytics •Supported technology transfer into closed and automated manufacturing platforms for scale-up readiness •Authored SOPs, protocols, reports, investigations, and documentation supporting regulatory submissions •Mentored junior scientists and collaborated with QA, manufacturing and leadership teams.

• Conducted QC tests for cell therapy products (CAR-T/TIL) in a GMP-certified facility, ensuring GMP compliance • Rigorous testing of clinical products using flow cytometry, ELISA, qPCR, vector titration, sterility/mycoplasma/endotoxin detection • Managed a team of executives • Subject matter expert with troubleshooting skills • Data analysis and presentation during meetings • Managed and assisted in projects within the R&D pipeline and worked on documents for HSA certification and IND application • Engaged with potential clients to explore and establish opportunities, contributing to the development of a sustainable business model • Completed technical knowledge transfer from counterpart to local site • Worked closely with production and QA team to streamline process

- Department of Virology (HIV and Zika virus) - Analysis of cell-associated sexual transmission and dissemination of HIV-1 and Zika virus in a human-cervical explant model - Expertise in laboratory techniques related to sterile cell culture - Working familiarity with laboratory techniques related to molecular cloning including restriction digestions, propagation of plasmids, transfections of cell line - Competent in Explant models, Histology, Immuno-histochemistry, Flow Cytometry, ELISA, blood purification - Experienced in working with BSL2 and BSL3 agents - Meticulous in record keeping as well as written and oral communication skills. - Competent with laboratory safety protocols related to operations of a research laboratory - Experience with laboratory management

-Lead Research Assistant for National University Hospital, Khoo Teck Puat Hospital and Alexandra Hospital based in Tan Tock Seng Hospital (Communicable Disease Centre - Singapore Infectious Disease Clinical Research Network). - Coordinate and administer clinical research studies research according to good clinical practice guideline (SGGCP) - Liaison between subjects and other members of research team - Recruit, coordinate and schedule study subjects for study visits as well as ensure compliance with schedules of evaluation and continuation of follow up by study subjects - Preparing study protocols, Institutional Review Board application, study site start-up. - Screening and recruitment, consent taking, retrieval of data from medical records, data entry, schedule subjects for follow-up visit and ensure compliance with study protocols. - Develop and maintain database system with proper documentation. -Coordinate application for funding and ethics approval and submit reports to the relevant authorities

•Experienced in trial monitoring and reviewing, in compliance with GCP and GLP regulations •Start-up development for innovative studies: o Researched on publications to minimise error o Proactive in searching and engaging specialists to seek expert advise •Ensure successful and timely execution of projects through meticulous planning (e.g. hosting start-up meetings, enforce periodical updates, drafting of SOPs etc.) among multiple parties: Directors, clients, veterinarians, chemists, medical technologists and technicians. •Involved in Institutional Animal Care and Use Committee discussion meetings •Client focused approach to work with the ability to interact professionally within a client organization •Exposed to multiple disciplines: Neuroscience (CSF, Parkinson’s Disease), Cognition (Object retrieval), Pain and Inflammation (L7 spinal ligation), Asthma and Abuse Liability. •Developed business opportunities through showcase of successful trial on innovative study •Effective time management to meet daily metrics or team objectives •Provided recommendations on In-Vivo parameters (dosing, sampling) •Competent in generating SOPs and consulting clients on study protocols •Generated unbiased reports with consistent accurate data from internal SDV •Prioritize multiple tasks and achieve project timelines •Co-authored poster publications •Client includes: Merck, Pfizer, GSK, BIAL, Grunenthal, Cephalon etc.

•Experienced in performing calibration, working and handling of LAFCs and Isolators •Compounding pharmacy products (Chemos, TPN, RTUs) under aseptic conditions (compliant with cGMP)

•Processed and evaluated drugs via Humidity Chambers and KF titration; with reference to post-testing parameters e.g. hygroscopicity, gas liberation etc •Generated reports for clients •Conducted Material Analysis using FTIR and WVTR on drug packaging materials

Modeling hereditary diffuse leukoencephalopathy with axonal spheroids using microglia-sufficient brain organoids Stem cell/Cell culture/ Organoids/ Co-culture/ Gene Editing