Wayne Gatlin

• Humanized chimeric mouse antibody and camelid nanobody molecules • Developed nanobody-enzyme-Fc fusion protein screening and expression platform • Created methods for expressing and purifying a bi-specific fusion protein with enzymatic activity • Developed automated four-step purification methods for in-house protein production • Engineered loss-of-function mutants for several bioactive molecules • Consulted with CMC on process development for lead molecule approaching Phase 1 trials • Developed HPLC methods for protein characterization

• Project co-lead in campaign to nominate new molecule for enzyme-antibody fusion protein • Engineering and cloning antibodies and new fusion protein formats • Developed protein purification methods to support multiple lab users and groups • Automated three-step purification workflow for lead molecules by building a custom AKTA system • Developed assays for PK/PD to characterize enzyme in mouse and monkey in vivo experiments • Authored methods for HPLC, Octet binding, ELISA, and more • Expressed proteins using HEK, CHO, and bacterial systems at discovery and laboratory scale • Discovered novel transfection feeding strategy that boosted enzyme activity of lead molecule • Contributed to publications, posters, and presented results at company meetings

• Expressed and purified recombinant kPAP2 enzyme from Trypanosoma brucei • Developed rRNA depletion probes and methods for Trypanosoma systems • Sequenced Leishmania EEP1 gene using custom 5’ RACE techniques • Trained undergraduate workers in the laboratory

• Managed science laboratory facility with a focus on biotechnology and bioengineering • Oversaw next-generation sequencing facility featuring Illumina MiSeq and Nextseq • Provided troubleshooting support and maintained laboratory equipment • Ordered supplies and managed inventory across twelve laboratory spaces • Coordinated and prepared weekly undergraduate laboratory exercises • Trained staff and students on proper use and care of laboratory machines and instruments • Taught lecture and laboratory courses in biology, biotechnology, and chemistry • Supervised a staff of student workers and facilitating adjunct instructor training • Acted as laboratory safety officer and primary author of laboratory safety policy