Rachel Wang

Key contributor to the progression of Biologics programs through antibody generation, characterization, and validation by functional assays Core team member of fibrosis and immuno- inflammation projects, coordinate Biologics activities cross-functionally and globally, research and evaluate literature to form protocols and strategies, present results on project team meetings. Developed and established functional assays used in generation and evaluation of reagents, assessment of mechanism of action and potency of lead and surrogate antibodies, resulting in the rapid progression of high-priority biologics projects within aggresive timelines. Antibody generation through hybridoma and phage display techniques. Screening arssay by ELISA, FMAT, FACS. Support Biotherapeutic projects through cell line generation antibody sequencing, assay development, fluorocytometric analyses (FACS), competition ELISAs, cell line receptor density assay Antibody purification by AKTA chromatography system, and characterization by ForteBio, SDS-PAGE, Bradford Assay, LAL Chromogenic Assay for Endotoxin testing

Major contriibutor of protein production platform to support high throughput screening, structure biology and assay development, characterization of proteins by analytical tools. Protein purification by affinity, ion exchange, gel filtration chromatography; expression of recombinant proteins in Baculovirus, mammalian and E. coli expression systems. Large scale protein production by Wave Bioreactor. Protein characterization by N-terminal sequencing, Agilent Bioanalyzer, analytical HPLC and Densitometer system. Develop and implement expression and purification protocols based on literature reviews and scientific experience; represented protein production group at project team meetings, presented all data which impacted project decisions

Coordinate research project of biologic, molecular, and genetic studies of human gliomas • Cloned gene of interest into appropriate vector; isolated DNA and mRNA from tissues and cells, as well as amplified DNA by PCR and purified PCR fragments • Identified gene mutation by sequencing and microsatellite polymorphism analysis; established primary cell culture and maintained stable cell lines ; tested the effect of potential drugs on tumor cell lines • Conducted protein extraction and quantitation and analyzed using SDS-PAGE, Western-blot analysis and Fluorescence In-situ hybridization • Developed and maintained a brain tumor database, entering and retrieving patient information as required; coordinated with researchers to access the database and patient specimen; through access to Cerner system, obtained patient pathology reports and communicated information with necessary individuals • Oversaw inventory and routine operations of the research laboratory; supervised and trained new personnel

Manage research project of in vivo analysis of developmentally regulated alternative splicing of the cardiac troponin-T (cTNT) gene • Cloned and screened various constructs of the cTNT gene to investigate the positive elements in the process of splicing • Designed and performed site-directed and oligonucleotide-mediated mutagenesis experimentsts to study the mechanism and regulation of RNA splicing; • Maintained mammalian cell lines and prepared primary tissue culture for transient and stable transfections • Purified and assayed RNA by primer extension and Northern-blotting; developed and implemented RT-PCR to improve sensitivity of RNA assay