Vivian (Wei) Guo
Senior Scientist @ Juvena Therapeutics
About
A passionate and innovative scientist with broad expertise across the biopharmaceutical and biotech industries, specializing in protein science and therapeutic antibody development. Highly skilled in protein biochemistry, molecular and cell biology, immunology, and immunoassays, with a strong track record of success from early discovery to preclinical development. Deep technical proficiency in protein expression, purification, conjugation, and characterization, as well as immunoassay and cell-based assay development. Proven ability to lead cross-functional projects, troubleshoot complex technical challenges, and deliver high-quality therapeutic candidates. CORE COMPETENCIES• Protein Design & Bioinformatics: Expert in protein and antibody engineering, sequence design and epitope prediction using AI and bioinformatics tools.• High-titer Expression Systems: Extensive experience with mammalian (expi293, expiCHO, CHOZN, CHOK1), insect (SF9/Baculovirus), and bacterial (E. coli) systems. Scaled transfections from microliters to liters, including stable cell lines generation.• Conjugation & Bispecific Assembly: Advanced in antibody/protein conjugation techniques (ADCs, peptides, DNA, small molecules, fusion proteins) and bispecific antibody engineering using linker chemistry for site-specific conjugation and heterodimer assembly.• Protein purification & Quality Control: Mastery in multiple purification methods (Affinity, SEC, IEX, HIC, TFF) from microgram to gram scale. Consistently deliver high-purity, low-endotoxin proteins for in vivo/in vitro use. • Antibody & Protein Characterization: Design and implement of protein & antibody stability study, and Developability Manufacturability Assessment. Techniques included but not limited to SDS-PAGE, HPLC-SEC, RP, LC-MS, DSC, nanoDSF, DLS, CE-SDS, WCX-HPLC, SCX-HPLC, endotoxin assays, ELISAs, cell-based assay, IHC, IF, Flow-cytometry, etc.• Therapeutic Antibody Development: Professional development of monoclonal abs, bispecific abs, humanized abs. Immunization strategies (design of potent antigens, highly conserved sequence and self-proteins). Proficient in hybridoma electrofusion, B cell cloning, antibody isotyping, subcloning, etc. Professional troubleshooting skills.• Immunoassay & Cell-Based Assays: High-throughput ELISA, Flow Cytometry, Octet, multiplex Luminex, GTPase activity, immunostaining assays.• Automation & High-Throughput Workflows: Liquid handling (Bravo, Beckman FXP/NXP) and methods programming.
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United States
Biotechnology
Therapeutic Protein and Antibody Development, Protein Biochemistry, Characterization, Biospecific Assembly , ADCs Conjugation , ADCs Stable Cell Line Generation, DNA prep, Clone Assay Screen and Clone pick, Clone Subclone, Protein Purification, Protein biochemistry, Protein characterization. , Protein Engine , Assay Development (HTP)
Experience
Senior Scientist
Juvena Therapeutics
Redwood City, California, United States
Protein Science • Led lead optimization for promising protein candidates, optimizing activity, stability, and pharmacokinetics. • Designed protein constructs and developed/optimized upstream and downstream expression methods. • Conducted high-throughput expression and purification (96/24-well plate, shaking flask, wave bag). • Performed biophysical characterization: GTPase activity, immunostaining, SDS-PAGE, HPLC-SEC, DLS, MS, endotoxin assays. • Contributed to early discovery and preclinical development to advance pipeline projects. Deep troubleshoot. SOPs creation.
Scientist II
Nektar Therapeutics
San Francisco Bay Area
Protein Therapeutics, Protein Chemistry • Designed and executed protein purification protocols for biologic therapeutics. • Evaluated transfection efficiency, construct optimization, and antibody purity. • Performed protein characterization: SDS-PAGE, HPLC, DSC, DLS, MS. • Established high-throughput antibody purification and AKTA/HPLC analytics. • Presented project updates to senior leadership.
Scientist
iBio, Inc.
San Diego, California, United States
Protein Purification and Analysis • Developed high-throughput protein expression and purification infrastructure from scratch. • Led antibody screening and plant-based and mammalian-based antibody purification projects. • Conducted protein characterization and analytical method development. • Supported business development and marketing initiatives.
Associate Scientist
Ambrx
San Diego, California, United States
Biologics ADCs stable cell line generation • Generated stable cell lines for ADC development using electroporation. • Conducted ELISA, Octet, and clone screening.
Associate Scientist
NGM Biopharmaceuticals
South San Francisco, California, United States
Protein Chemistry, Antibody Development • Purified and characterized ADCs, bispecific antibodies, monoclonal antibodies, chimeric antibodies, therapeutic humanized antibodies and therapeutic proteins. • Developed bispecific antibodies assembly, ADCs conjugations. • Designed immunization strategies, performed protein conjugation and peptide modifications. • Developed hybridoma laboratory and automated clone picking, HTP assays. • Supported multiple Phase II clinical projects and contributed to two publications (2021).
Associate Scientist
Biocare Medical
Concord, California, United States
Antibody Research and Development • Designed protein structures and performed rodent immunization strategies. • Conducted hybridoma and B cell cloning for mAb generation. • Managed lab operations, trained colleagues in antibody development techniques.
Scientist III
Life Technologies
Camarillo, California, United States
Antibody Development, Assay Development • Developed ELISA kits, multiplex Luminex assays, and hybridoma isotyping kits. • Designed B cell cloning strategies, aiming for 1000 antibodies/year. • Led automation projects for high-throughput workflows (Beckman FXP/NXP). • Supervised and trained team members in protein purification, conjugation, and antibody characterization.
Vivian (Wei) Guo's Contact Information
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