valerie liao

manufacturing cell culture technician

About

I am a small scale CHO technician looking to progressively augment my skill set in the biotech industry. My experience up until now has mostly been in mammalian cell culture (CHO), but I would like to expand that by incorporating bacterial fermentation or solidifying assay techniques. Although ideally I would like to gain experience in each aspect of making a pharmaceutical drug, my experience up until now gives me a strong mammalian cell culture background. My goal is to continue refining my knowledge of cell culture and learn more about the industry.

Country

united states

City

san francisco

Industry

biotechnology

Skill

molecular biology, recombinant dna technology, laboratory, aseptic technique, technician, biotechnology, research, manufacturing, cell culture, bioreactor, mammalian cell culture, fermentation

Experience

research associate i

catalyst biosciences

2011-9 - 2012-4 · 8 mos

* Mammalian cell culture of CHO and BHK in shake flasks and WAVE bioreactors * Transient and stable expression of recombinant proteins * Growth, expression, harvest, and depth filtration of cell culture in 5L and 10L scales * Generation of stable lines from transiently transfected culture * Media development for CHO stable cell lines * Sterile technique in hood and open environment

manufacturing cell culture technician

genentech

2013-4 - Present · 13 yrs 6 mos

pilot plant technician

genentech

2012-8 - Present · 14 yrs 2 mos

* CHO cell culture in shake flask, 2L, 10L, and 20L fermentor scale * Daily sampling of CHO experiments * Working closely with technician specialists and researchers to troubleshoot in-process experiment discrepancies * Working closely with various vendors to design an efficiency item * Manage traffic of warm room sterility samples * NovaFLEX maintenance, when needed

Education

university of california, merced

ecology

2007-1 - 2011-1 · 4 yrs 1 mo

Activities and Societies: Taiko Captain (2010-2011) for the Nikkei Student Union, Secretary (2009-2010) and Vice President (2010-2011) for the Anime Club, Inter Club Council representative (2009-2011).