Sydney Fronck

- Set-up, operate, clean and break down of gel column chromatography and skid, TFF skid, FIT and in-process testing equipment (pH meter, conductometer, spectroscopy) to perform manufacturing duties in stainless steel 20K production facility - Adhere to SOP, WI, batch record, site safety, site quality, cGMP, GDocP, FDA standards, data integrity standards - Complete cleanroom changeover procedures, logbook review, batch record review - Aseptic open connections and sampling techniques (communicate with QC per procedure to submit samples) - Work with buffer prep, glasswash, production planners to ensure materials are tracked and available when needed - Collaborate with other groups onsite as necessary

- Aseptic mammalian culture in shake flasks within BSC, wave reactors, SUBs to perform manufacturing duties in single-use 2k production facility; produce media and assemblies or use in-process testing equipment (Vi-Cell, Nova, pH meter, FIT) as needed - Adhere to SOP, WI, batch record, site safety, site quality, cGMP, GDocP, FDA standards, data integrity standards - Complete cleanroom changeover procedures, logbook review, batch record review - Creation, authorization and implementation of deviation investigation reports (involving RCA), CAPA projects, change controls - Generation and revision of SOPs, WIs, batch records, protocol and training plans - Train personnel on equipment, processes, techniques - Onboard new equipment (support engineering, MSAT, automation, metrology) - Process development support with media qualification study (led my team, created and managed schedule for all teams) - QA support with document archival - Communicate with supply chain and order materials to ensure materials are tracked and available

- Aseptic mammalian culture in shake flasks within BSC, wave reactors, SUBs to perform manufacturing duties in single-use 2k production facility; produce media and assemblies or use in-process testing equipment (Vi-Cell, Nova, pH meter, FIT) as needed - Adhere to SOP, WI, batch record, site safety, site quality, cGMP, GDocP, FDA standards, data integrity standards - Complete cleanroom changeover procedures, logbook review, batch record review - Creation, authorization and implementation of deviation investigation reports (involving RCA), CAPA projects, change controls - Communicate with supply chain and order materials to ensure materials are tracked and available

My final year, I completed an independent research project where I looked for antibiotic potential in the extremely hot soils of Centralia, PA, where a mine fire is burning. It is estimated that 99% of bacteria have yet to be cultured, so continuing to search this unique location will reveal new antibiotics which will allow us to face the crisis of antibiotic resistance. With this project, I worked with a team in the lab and then independently analyzed the data to write the final research paper. This project allowed me to gain experience in culturing bacteria on various media types, running gel electrophoresis, and completing PCR.

Provided a safe work environment for students working in biology laboratories in the evenings and on weekends by following safety policies.

Assisted 15+ students with lab techniques and protocol, made sure lab safety rules were followed, and graded notebooks and presentations.

I took immersive lab courses expanding branches of biology including ecology, cell/molecular biology, and organismal biology. The most exciting courses I have taken are Animal Physiology, Molecular Biology, and Organic Chemistry. My independent senior research project searched for antibiotic potential in the soil above a mine fire. This liberal arts education emphasizes an interdisciplinary understanding across subjects other than my major, so I have developed a variety of skills involving critical thinking and communication.

I absorbed the culture of New Zealand during my study abroad semester. Courses in the Māori language and the Treaty of Waitangi allowed me to connect with the history of the country and the cultures that have shaped New Zealand society.