sui-po zhang

* Performed bioassay development for compound screen and target validation, evaluated second generation compounds for further development. * Developed TRPV1 receptor binding assay to initiate a TRPV1 inhibitor project and a high-throughput screening method for N-type calcium channel blockers * Characterized the voltage-dependent calcium channelalpha2/delta1 subunit in KO mice * Investigated voltage-dependent calcium channel alpha2/delta1 subunit in IMR32 cells * Evaluated gabapentin in calcium mobilization and imaging assay * Developed COX activity assays using EIA, colorimetric and chemiluminescent assays to screen and identify lead compounds * Characterized cloned human COX-3 to evaluate the target * Received a JNJ Vision Award for solving assay problems * Promoted to Scientist position

* Served as a laboratory manager and conducted research on calcium-binding proteins and neurotransmitter receptors; performed enzyme assays, receptor bindings, animal behavior testing and cell culture, studied gene regulations. * Studied the interactions of drugs with CaM and CaM-sensitive enzymes * Investigated structure-activity relationships of CaM antagonists with a florescence method * Investigated CaM gene regulation in PC 12 cells using CaM antisense and measured the mRNAs levels by in situ hybridization cytochemistry using [35S] labeled probes, Northern blot analysis and RT-PCR * Quantified the receptors by binding assay and receptor autoradiography * Evaluated animal behaviors including turning, grooming, rearing and locomotion * Investigated effects of neuroleptics on dopamine receptor mRNAs, enkephalin mRNA and GAD mRMA using in situ hybridization histochemistry and Northern blot analysis

* Pharmaceutical Development and Manufacturing Sciences * Bioassay Method Development * Bioassay Method Qualification * Bioassay Method Validation * Bioassay Method Transfer * Project Representative * In vitro Pharmacology

* Performed drug discovery research on analgesics, served as a project leader to conduct bioassay development for compound screen and target validation, evaluate lead compounds for preclinical study, recommend moving forward compound to next stage. * Evaluated directed synthesis compounds in a fluorescence based enzyme assay * Developed an ex-vivo assay to evaluate lipase inhibitor and a cell based lipase enzyme activity assay * Investigated the lipase product pathway by siRNA * Characterized lipase knockout mice to evaluate the target * Received a JNJ Encore Award for validating new target * Identified compounds for PK,PD and safety studies * Led one project from target proposal to lead generation, lead optimization and early development

* Conducted drug discovery research on analgesics, tested primary and second generation compounds, analyzed data and completed all documents. * Developed opioid receptor functional assays with GTPgammaS as a ligand to identify agonists and antagonists and bradykinin receptor binding assays for screening and testing compounds to identify lead compounds * Characterized bradykinin receptors in human lung fibroblasts * Developed a calcium mobilization assay to confirmed lead compounds for GPCR targets * Promoted to Senior Associate Scientist position