Shing-hong Lin

Highly motivated, independent, and experienced pharmaceutical Scientist Great Boston, MA 02459 shinghonglin@gmail.com 6177634567 My expertise is in mouse genetic, I can perform techniques ranging from molecular cloning, in vitro culture neuron characterization, ex vivo tissue preparation to in vivo mouse disease model (see my CV, request by messaging); recent progress in Harvard Neurobioloogy department are focus on dissecting spinal circuits mediating acute and inflammatory visceral/cancer pain using mouse cancer/colitis model including DSS, TNBS, Zymosan and cancer cells transplantation. I am an organized, adaptable, creative, excels at multitasking, and experienced with prioritizing tasks and projects management. Driven to achieve deliverables in a timely manner. Excels at technical troubleshooting and protocol development, validation, and optimization. Experience presenting results at conferences and team meetings, providing subject matter expertise to project team, and being an intellectual contributor to group discussions. Excellent written and verbal communication skill in both Chinese and English. Skills: •Mouse genetic engineering technique (CRISPR/TALEN/AAV/Lentiviral) •Mouse behavioral phenotyping (proprioception, nociception, addiction, affective disorder and learning & memory task) •Molecular cloning (Viral vector construct, DNA/RNA/protein characterization) •Electrophysiological recording in DRG neuron (single cell patch) •Mouse brain, spinal cord stereotaxic surgery (cannulae, optic fiber, ICV, lesion, AAV injection) •Mouse cancer and Colitis inflammation model (DSS, TNBS, Zymosan) •Optogenetic/Chemical genetic techniques (in vivo blue light-, CNO manipulation) •Ca-imaging (DRG neurons)/EMG recording (Colorectal distention) •Fluorescence/Confocal microscopy/flow cytometry (+10 years experience) •Immunohistochemistry & in situ hybridization (neuron, glia, muscle. colon, liver and skin tissues) •High-Performance Liquid Chromatography (Catecholamines, amino acids) •in vivo microdialysis in rodent brain •Experimental design and Statistics (SAS, SPSS) •immunoassay (antibody engineering, ELISA, IHC, in situ hybridazation) •Southern, Northern, Western Blot •Realtime/RT-PCR/digital PCR •Psychopharmacology (rodent learning & memory, addiction, fear, anxiety, depression, autism models) •Primary and iPSC cell culture •PK/PD/Biomarker/Tox study with CRO •ELN