Sebastian Espinoza-Ulloa

As R&D Manager at the North American Insect Center (NAIC), I lead phenotyping, selective breeding, and genomics projects focused on Black Soldier Fly (BSF). My role includes managing laboratory rearing, refining breed varieties, and collecting detailed phenotypic data for molecular analyses. I also develop automation solutions (hardware and software) to enhance efficiency, alongside creating and implementing SOPs for standardized processes. Leveraging bioinformatics and Whole-Genome Sequencing (WGS), I drive genetic insights that improve breeding strategies and operational outcomes.

I am leading initiatives in phenotyping, selective breeding, and genomics applied to insects (particularly BSF). My role involves comprehensive laboratory settlement rearing, defining breed varieties, and articulating phenotypic traits. I implement standardized rearing protocols and collect detailed phenotypic data while preparing tissues for molecular analyses. Additionally, I contribute to the development of cutting-edge automation solutions, including both hardware and software, and engage in programming and software development to enhance automation efficiency. I also craft Standard Operating Procedures (SOPs) to ensure systematic processes and apply bioinformatics and data analysis techniques to advance our understanding of breeding genetics from Whole-Genome Sequencing analyses.

I teach and have taught courses in the Health Sciences and Psychology faculties, including Principles of Biochemistry, Biology Introduction, Molecular Techniques for Medical Diagnosis, and Scientific Research Methodology. My responsibilities encompassed annual teaching planning, class and activity preparation, and the development of assessments. I also focused on research education, training students to search for scientific information, and developing research projects.

I worked on the project "Systematics and Diversity of Neotropical Amphibians and Reptiles: Phase I" at PUCE. My duties included DNA extraction from tissue samples of various organisms such as plants, reptiles, and amphibians, and quantification of the obtained DNA. I performed PCR amplification of conserved genes and loci, standardizing and adjusting PCR protocols to achieve optimal results. I also developed new PCR protocols for additional genes and loci. My role involved testing the purity and concentration of PCR amplification products using electrophoresis on agarose gels, preparing PCR products for sequencing, and conducting quality reviews and troubleshooting of sequences. Additionally, I was responsible for the registration and processing of databases.

I contributed to the SENECYT-PUCE project titled "Genetic Characterization and Bioinformatic Analysis of the Diversity of Podocarpus, Sangay, Yacuri, and Llanganates National Parks" under the Arca de Noe (Noah's Ark) initiative. My responsibilities included DNA extraction from tissue samples of various organisms and quantification of the obtained DNA. I performed PCR amplification of conserved genes and loci, standardizing and adjusting PCR protocols to optimize results. I developed new PCR protocols for additional genes and loci, tested PCR amplification products for purity and concentration using electrophoresis on agarose gels, and prepared PCR products for sequencing. My work involved obtaining PCR products from different organisms and genes, resulting in about 800 sequences for analysis. Additionally, I conducted quality reviews and troubleshooting of sequences and managed the registration and processing of databases.

My experience included conducting fieldwork and sampling, tissue preparation for molecular analyses, and the preparation and handling of solutions and reagents. I performed PCR, library preparation, and sequencing, followed by mapping, aligning, variant calling, and SNP filtering. My work also involved advanced bioinformatics and data analyses, sequencing, De novo assembly, and genome annotation. Additionally, I contributed to scientific publications, showcasing the breadth and impact of my research.

My role involved preparing and demonstrating laboratory material, tutoring students, and assisting with various activities. I moderated discussion boards, hosted virtual meetings via WebEx, and took on responsibilities such as marking, invigilating, and delivering briefings.

I worked on the PRAS (Programa de Reparación Social y Ambiental) and SIPAS (Sistema de Indicadores de Pasivos Ambientales y Sociales) projects. My responsibilities included developing Environmental and Social Liabilities Indicators and conducting statistical analyses on a regional scale. I created interaction webs linking economic activities with their liabilities, developed reparation plans, and monitored and evaluated these plans. Additionally, I reviewed data and technical reports, prepared technical reports, and disseminated public reports.

I contributed to the "Santa Elena and Refinería del Pacífico" project. My duties involved conducting entomological samplings, designing statistical evaluations, and analyzing biodiversity and habitat quality. I determined areas by biotic sensitivity and assessed the environmental impact on regions designated for oil exploration. Additionally, I mounted museum specimens and prepared government reports.

I played a key role in BioMol Ecuador Limited Company, specializing in equipment and reagents for molecular biology, research, forensics, and health sciences laboratories. I provided comprehensive support, including equipment installation, counseling on reagents and techniques, and technical assistance. Additionally, I actively contributed to sales operations and billing processes, ensuring effective client engagement and satisfaction.

I oversaw various aspects of operations to enhance guest experiences and operational efficiency. My responsibilities included providing exceptional customer service, identifying strategic investments, managing financial resources, overseeing personnel, advocating for conservation efforts, and promoting the lodge to increase visibility and attract visitors.

I led a butterfly farm dedicated to commercial and tourist purposes. Located in an ecological setting, my responsibilities encompassed a wide range of activities crucial to the farm's success. I focused on species reproduction both in situ and ex situ, ensuring sustainable populations. Additionally, I managed butterfly mounting and species identification processes, implemented pest and disease control measures, and conducted comprehensive business research and planning. I developed breeding protocols to optimize production efficiency and quality. Furthermore, I conducted research on end products derived from the farm and oversaw ecological management practices to maintain environmental sustainability.

I contributed to the project "Inventory and Characterization of genetic and morphological diversity of amphibians, birds, and reptiles from the Andes of Ecuador." My responsibilities included extracting DNA from various species and individuals, standardizing PCR protocols, and amplifying several genes of taxonomic importance. I also prepared samples for sequencing and played a key role in the registration and development of databases to manage research findings effectively.

My responsibilities included conducting microbiological quality control analyses on raw materials, products, equipment, environments, and personnel. I specialized in culturing and identifying microorganisms such as bacteria, yeasts, and fungi, including standard (reference) microbial strains, both pathogenic and non-pathogenic. I played a crucial role in creating and reviewing reports within jurisdictional areas, as well as standardizing and reviewing protocols for managing strains, microbiological sampling, and equipment use to ensure stringent quality control standards were met.

As part of S&R Consultant Company, I was responsible for implementing standard operating procedures (SOPs) to ensure environmental compliance. My role involved conducting comprehensive reviews of reports, analyzing data collected through thorough assessments, and compiling precise and objective outcomes for regulatory purposes.