Rutuja Kulkarni

Core pathology Lab at DCMC Maintains safe and clean working environment by complying with procedures, rules, and regulations. Labels and disposes biohazard and/or chemical waste appropriately. Maintains inter- and intradepartmental work flow by fostering a spirit of cooperation (hand offs). Maintains laboratory supplies inventory by checking stock to determine inventory level; anticipating needed supplies; placing and expediting orders for supplies; verifying receipt of supplies. Conserves laboratory resources by using equipment and supplies as needed to accomplish job results. Determines and executes the best method for obtaining biological specimens from patients of all ages as required to include venipuncture, capillary, and heel-stick puncture to ensure quality specimens for testing. Processes gynecologic and non-gynecologic specimens for microscopic evaluation. This includes instrument processing, staining, changing reagents, and cover-slipping. Performs functions to established productivity guidelines with minimal errors. Execute Laboratory Information System (LIS) Prepares aliquots and processes samples for referral to outside labs; distributes specimens to testing or processing locations according to established standard work. Operates and maintains laboratory equipment. Performs preventative maintenance and troubleshoots problems to the full extent of ability Participate in the training of new staff and students by serving as a mentor and technical resource.

• Maintained and cultured the mammalian cell line using advanced aseptic techniques, ensuring optimal cell growth • Executed and analyzed Cell-Based Potency Assays ELISA and MTT, delivering precise data interpretations. • Monitored cell survival post-treatments, ensuring data validity by using flow cytometry • Performed titration and prepared potent bacteriophage stock tailored for specific E. coli strains. Led bacteriophage experiments targeting E. coli strains, both in vitro and in vivo • Actively assisted undergrad students in their academic research presentation, guidance in DOE, execution, and data analysis • Utilized Microsoft Office, Microsoft excel, JMP for streamlined data analysis and reporting. • Collaborated closely with a diverse team of researchers, students, and faculty in research based on immunology

• Conducted Human semen analysis, DFI, vitality test,semen morphology, preparation of semen samples for ICSI, IUI, IVF • Executed oocyte pickups, Performed Embryo transfer, Embryo biopsy • Interpretation, and analysis of clinical reports of Biopsy study and maintained records using Excel, AI tools • Performed Cryopreservation of human semen, human egg, and human embryo. Maintained the records and data entries of patients and assisted in maintaining GLP and GCP in accordance with standard regulation • Collaborated with the team of gynecologists and researcher to study and interpret infertility causes, biopsy study analysis Conducted independent research on various infertility issues in male and female

• Efficiently processed laboratory specimens, Human semen sample for vitality test, IUI, semen morphology • Prepared of blood smearsfor hematology tests by centrifugation, aliquoting. • Meticulously labeled cryovial tubes, maintaining accurate specimen identification and cryopreserved the samples • Evaluated and maintained laboratory records and patient clinical reports using data tools

• Assessed Human semen sample, DFI, vitality test, semen morphology • preparation of human semen samples for ICSI, IUI, IVF • Evaluated and maintained clinical reports using data management AI too