robert wisotzkey

Analyzed genetic variants to determine pathogenicity based on literature and allele frequency information following ACMG guidelines. Developed strategies for automating portions of the process. Reviewed genes for inclusion in targeted diagnostic panels. Developed standardized language for patient reports.

Teaching lecture and laboratory courses in Genetics, Genetic Analysis of Development, General Biology, Cell and Molecular Biology, and Evolution. Responsible for curriculum development, lectures and grading.

Analyze gene expression, genome copy number, Chromatin – IP and DNA methylation studies with standardized protocol and populate and curate a database with the results. Assisted other teams in the development of the procedures to analyze the genome copy number variation studies and ChIP-Chip studies. Germ line variant curation.

Populated internal databases of proteins that are potential small molecule drug targets, secreted protein therapeutics or antibody targets. Responsible for determining orthologous relationships between mouse and humans. Managed research associates.

Identified Caenorhabditis elegans homologs of mammalian proteins of interest by analyzing the C. elegans genome. Also analyzed the C. elegans genome for genes with particular enhancer elements to identify potential new targets.

Positionally cloned and characterized the Drosophila gene Medea. Sequenced mutant alleles to confirm the transcript isolated encoded the gene of interest. Examined the role of Medea in the embryonic development using germline clones as well as homozygous mutant larvae.