Renjing Wang

Memorial Sloan Kettering Cancer Center and Howard Hughes Medical Institute Postdoc and Senior Scientist PI: Nikola P. Pavletich Research Focus: Structure/Function of proteins in DNA Interstrand Crosslink (ICL) Repair by crystallography and Single-Particle cryo electron microscopy Achievements: ● Achieve 2 publications in top journal Science and Nature as a leading author ● Train and supervise 2 technician and 5 graduate students in protein purification, enzymatic assays and pursuing protein structure determination and data analysis ● Collaborate with 3 labs, co-develop a technique to synthesize ICL DNA using click chemistry which is in preparation for publication ● Determine novel protein/DNA complexes structures using X-ray and single particle cryogenic-EM . Extensive experiences in protein characterization from cloning to structure determination and function study ● Manage the mosquito crystallization robot system and associated supplies

PI: Chongwoo A. Kim, Ph.D. Research Focus: Structure and function study of polycomb group proteins in epigentics Achievement: A total of seven publications, and four of which I am the first author ● Construct and express large-scale protein for high-throughput crystallizaion of single protein and protein complex to uncover the mechanism of how the polycomb group complex repress gene expression cooperatively. ● Extensive experience for purifying protein from inclusion body and refold it in affinity column or in solution. Proficient in HPLC for affinity, IEX, RPC and SEC ● Determine multiple crystal structures of protein complexes of Polycomb Repression Complex (PRC1) proteins involved in epigenetic regulation ● Solve solution structure of hPh1 FCS domain using NMR ● Transcription repression assay in Drosophila S2 cells ● Characterization of protein-protein, protein-RNA and protein-DNA interactions using EMSA, AUC, SPR, NMR and in vivo FLIP-FREP