rajeev kumar

A) Established an anti CD19 Chimeric Antigen Receptor (CAR) T cells for hematologic malignancies. B) Developing a method to restore impaired effector function of CD8+ T cells using advanced genome editing (CRISPR/Cas9) technology. C) Prepared universal iPSCs using CRISPR/Cas9 technology.

Study of anti PD-1 off target responsible for tumor escape from CD8+ T cells based adoptive immunotherapy, Tumor metastasis detection in sentinel nodes from colorectal cancer patients by using FACS multiple parameter assays- technology and clinical application, Pharmacodynamic investigations of therapeutic T cells in xenograft tumor mice.

I attempted to define newer management strategies for the prostate diseases, BPH and CaP, using the approaches of phyto-therapy as well as designed drugs (chemotherapy for hormone refractory prostate cancer). In phytotherapy we have identified effective lead in seeds of a cucurbitaceous plant for the management of BPH while some phytochemicals in unique combinations synergistically inhibited CaP cell proliferation. On the other, a number of molecules designed as SERMs exhibited promise as new management agents for BPH and CaP. For in vitro experiments I used human BPH-derived primary stromal cell cultures and CaP cell lines. For in vivo, experiments adult Sprague Dawley albino rat model was used. With the aid of molecular and cell biology techniques, I resolved the signaling pathways and other parameters involved in the action of promising agents. Role of estrogen receptor (beta), MAPK signaling and PI3K/Akt pathway has been implicated in the action of various new agents.

Summer training: 1. One month training at Auroprobe Laboratory: Mycobacterium tuberculosis isolation in GLP laboratory. PCR based Tuberculosis and HPV diagnosis. 2. Short dissertation at Sanchi Dairy, Gwalior, India “Isolation and characterization of pathogenic bacteria from Milk”.

Dissertation topic: “Isolation of bioactive compounds from associated sea microorganisms”, National Institute of Oceanography (Council of Scientific and Industrial Research), Dona Paula, Goa, India. This course covered principles and applications of microbiology and genetic engineering in the field of drug discovery research. Activities and Societies: Attended Indian Science Congress held at Chandigarh (January 2004), National symposia on Recent Trend in Biomedical Science at Bundelkhand University (2003).