Rachael Lucero, Ph.D.

-Designed, executed, and evaluated lead discovery screening methods and data to identify compound hits and advance them through hit-to-lead and lead optimization stages to support screening and preclinical development -Collaborated within department and other teams (Med. Chemists, Compound Management, Biology, Structural biologists, Computational) to develop and implement biochemical and biophysical assays for hit identification and lead optimization, evaluating compound potency, selectivity, and mechanism of action, biophysical and biochemical assay development

Scientist - Assay Technology (Lead Discovery) for Revolution Medicines Supporting Discovery Science in the search for new oncology therapeutics Designing and executing biochemical/biophysical assay development for screening methods to identify and optimize compound hits

Independently developed and executed a research plan to investigate the biochemical, biophysical electrophysiological, and structural properties with xenobiotic substrates of bacterial small multidrug resistance (SMR) membrane proteins found in wastewater Characterized proteins using recombinant expression and purification, reconstitution into liposomes, solid-supported membrane electrophysiology, Michaelis-Menten kinetics, ITC, tryptophan binding assay, X-ray crystallography providing the first evidence into bacterial co-option of existing metabolic transporters to deal with novel xenobiotics Quantified bacterial fitness by competing labeled knockout and non-knockout strains with potential toxic metabolic substrates by analyzing through growth, resistance, biofilm, and flow cytometry assays Performed statistical data analysis and publication-quality visualization using GraphPad and Adobe Illustrator Mentored undergraduates and manage scheduling, technical training, note-keeping and project development Disseminate research at lab meetings, conferences, and peer-reviewed journals Completed multiple projects ahead of deadlines and regularly disseminated results in yearly committee meeting and monthly lab meetings

Collaborating with Dr. Alberto Rascon under San Jose State University's Department of Chemistry. Thesis Title: “A Biochemical Study of the Autocatalytic Nature of a Serine Protease (Early Trypsin) from the Aedes aegypti Female Mosquito” Characterized difficult-to-express mosquito metabolic proteins using molecular cloning, PCR, mutagenesis, bacterial protein expression of soluble recombinant proteins, and Western blotting Purified proteins via Fast Protein Liquid Chromatography (FPLC) and quantified enzyme kinetic parameters via chymotrypsin and trypsin enzyme kinetic assays

Teaching Associate for: CHEM 1A (General Chemistry) Laboratory CHEM 131B (Advanced Biochemistry) Laboratory Developed and executed lecture materials and experimental demonstrations Instructor of Record, sole-taught lab (30 students) and lecture courses (120 students)

Held weekly meetings with fellow peer advisors to record any potential issues with students, instructors, or any outside commitments that may influence the work place Reported weekly to Senior Coordinators and Director of program about potential issues with instructors and/or peer advisors Held one-to-one meetings with freshman, transfer, or probational students to provide guidance for successful career in college

Collaborating with Dr. Alberto Rascon under San Jose State University's Department of Chemistry.

Collaborating with Dr. Luis Bonachea under San Jose State University's Department of Biological Sciences. Husbandry of crickets with daily cleaning, changes of food and water and monitoring well-being of the insects Analyzed changes in size of wind sensors in the offspring cricket through a scanning electron microscope (SEM) for insight of maternal effect mechanisms when exposed to chemical cues from a predator

Held weekly meetings with fellow peer advisors to record any potential issues with students, instructors, or any outside commitments that may influence the work place Reported weekly to Senior Coordinators and Director of program about potential issues with instructors and/or peer advisors Held one-to-one meetings with freshman, transfer, or probational students to provide guidance for successful career in college