Qiming Yang

• Led independent research on gene regulation and chromatin remodeling during X-chromosome dosage compensation in C. elegans using genomic, biochemical, and imaging-based approaches • Collaborated with researchers, technicians, and students to co-design experiments, troubleshoot protocols, and align on shared goals • Authored 3 peer-reviewed publications; presented findings through talks and posters at national and regional scientific conferences • Built reproducible analysis pipelines for ChIP-seq, RNA-seq, and Hi-C using Bowtie2, Samtools, DeepTools, and custom Python scripts Technical Experience (Wet Lab Focused) • Designed CRISPR/Cas9 workflows including gRNA design, plasmid construction, microinjection, and mutant screening • Engineered plasmids for gene expression, fluorescent tagging, and RNAi knockdowns • Performed ChIP targeting histone modifications (e.g., H4K20me1) and regulatory proteins (e.g., DPY-27, SEX-1) • Conducted high-resolution imaging via IF and smFISH • Applied western blotting, co-IP, and pull-down assays for protein interaction studies • Purified high-specificity antibodies for use in ChIP, IF, and western blot • Conducted nucleic acid extraction (fragmented/genomic DNA) and quality control using qPCR/RT-qPCR and fragment analysis (TapeStation, FragmentAnalyzer) • Performed lentivirus-mediated transductions for protein expression, including engineered constructs and partial protein domains, in mammalian cell systems for biochemical studies. Technical Experience (Computational Focus) • Translated sequencing and imaging datasets into insights via analysis and visualization workflows • Built automated pipelines for Illumina (RNA-seq, ChIP-seq, Hi-C) and nanopore (DNA methylation, isoform detection) data, applying statistical methods to quantify gene expression, chromatin enrichment, and epigenetic modifications • Developed batch image quantification workflows using Imaris and Python • Experienced with Linux-based environments and Git

• Taught upper-division molecular biology and genetics; delivered lectures, mentored undergraduates, and supported students across in-person, hybrid, and remote formats—including during the COVID-19 transition • Substituted for faculty when needed and served as a liaison to manage course logistics, resolve student concerns, and ensure instructional continuity • Designed and graded problem sets, lab exercises, and exams aligned with course objectives; promoted analytical thinking and application of experimental logic

• Taught upper-division molecular biology and genetics; delivered lectures, mentored undergraduates, and supported students across in-person, hybrid, and remote formats—including during the COVID-19 transition • Substituted for faculty when needed and served as a liaison to manage course logistics, resolve student concerns, and ensure instructional continuity • Designed and graded problem sets, lab exercises, and exams aligned with course objectives; promoted analytical thinking and application of experimental logic

• Contributed to CRISPR/Cas9 genome editing workflows by designing gRNAs, constructing plasmids, performing microinjections, and screening mutants; presented research via posters at national and regional symposia • Optimized plasmids for gene expression and fluorescent tagging experiments • Performed western blotting, co-immunoprecipitation, and protein purification for protein interaction studies

• Performed forward genetic screens in C. elegans to identify phenotype-altering mutations • Gained foundational lab skills in cloning, media prep, and sterile technique