Da Zhang

Scientist @ Benitec Biopharma Inc.

About

I am currently a scientist at Benitec Biopharma, working on manufacturing and process development on AAV-based gene therapy. Before I joined Benitec Biopharma. I complete PhD degree in university of Arkansas where I have been working on the fouling detection, modeling, visualization during tangential flow filtration and alternating tangential flow filtration. Before joining University of Arkansas, my research is foam fractionation, which is a novel method for protein enrichment. I also have experience on molecular cloning and antigen expression as a research in the biotechnology industry. For more information and CV, please visit my personal website: dazhang.org. I am looking forward to connecting with you!

Country

United States

City

San Francisco Bay Area

Industry

Biotechnology

Skill

Microfiltration, C++, Molecular cloning, Python (Programming Language), Machine Learning, Cell Culture, Purification, SDS-PAGE, Modeling, Deep Learning, C (Programming Language), Perfusion cell culture, Tangential flow filtration , High-Performance Liquid Chromatography (HPLC), Real-Time Polymerase Chain Reaction (qPCR), Fast Protein Liquid Chromatography (FPLC), MATLAB, Cloud Computing, ELISA, Liquid Chromatography-Mass Spectrometry (LC-MS)

Experience

Scientist

Benitec Biopharma Inc.

2022-6 - Present · 4 yrs 3 mos

Hayward, California, United States

Graduate student Co-op

Biogen

2021-6 - 2021-9 · 4 mos

Cambridge, MA, United States

Graduate Student Co-Op

Amgen

2021-4 - 2021-6 · 3 mos

Massachusetts, United States

Student Intern

RAPID (AICHE): RAPID: Rapid Advancement in Process Intensification

2020-7 - 2020-8 · 2 mos

Cell harvesting the beginning of the downstream purification process for most of protein-based pharmaceutics. This process is more challenging because of the increase of cell density and cell culture duration, especially with the adaption of alternating tangential flow filtration(ATF) technique. The most limiting factor of this process is the fouling of the membrane. This work tries to answer 2 main questions: what is the foulants and how they fouled the membrane. Experimental works include the 2D SDS-PAGE and foulants identification by LC-MS. With a better understanding of the first question, the pharmaceutical manufacturer could eliminate foulant that is identified from this work. The second question could help the membrane manufacturer to design a better membrane with higher mass transfer.