purnima wagh
research associate
About
I am a Molecular and Cell biologists. I have expertise in Cloning, DNA and RNA extraction, miRNA and mRNA qPCRs and RNAseq, western blotting, northern blotting, flow cytometry, siRNA transfections and cell culture, primary breast and lung cell isolation, animal handling, drug/antibody testing in animal models (subcutaneous and orthotopic). Apart from this I also have used various software programs such as GraphPad Prism, TRAFAC and rVISTA.
united states
cincinnati
hospital & health care
western blotting, research, cell culture, lifesciences, elisa, molecular biology, biochemistry, animal models, pcr, cell biology, microscopy, immunohistochemistry, in vitro, qpcr, transfection, immunofluorescence, in vivo, flow cytometry, protein expression, cancer research, protein purification, immunoprecipitation, rt pcr, assay development, cell, protein chemistry, dna, cell signaling, fluorescence microscopy, scientific writing, immunocytochemistry, cancer, genetics, tissue culture, graphpad prism
Experience
phd
university of cincinnati
* Identified beta-catenin role in Ron Receptor induced mammary tumorigenesis * Determined if Conditional Deletion of beta-catenin in Mammary Epithelial Cells of Ron Receptor * Overexpressing Mice Alters Mammary Tumorigenesis * Determine the role of MSP/HGFL in Ron dependent mammary tumorigenesis * Determine the efficacy of MSP/HGFL neutralizing drug on mammary tumors derived from MMTV-Ron cells
research associate
cincinnati children's hospital medical center
post-doctoral fellow
cincinnati children's hospital medical center
* My research is to identify molecular mechanisms impacting lung development and disease * Performed mRNA and miRNA sequencing as well as proteomics to identify novel targets in pediatric lung disease * Developed in vitro assays to examine micro RNA regulation in lung tumors * Targeted gene disruption in murine model to identify role of novel oncogene in lung
Education
st. john the baptist high school
university of kentucky
chatrapati sahuji maharaj kanpur university, kanpur
biology
udct university institute of chem. tech
purnima wagh's Contact Information
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