S.K.Priya Pulya

•Designed and executed bioanalytical assays across multiple programs to support PK/PD and translational biomarker strategies for RNA therapeutics programs. •Developed and optimized immunoassays (MSD, ELISA, Luminex, AlphaLISA, HTRF) and RT-qPCR assays to assess drug exposure, target engagement, and gene knockdown. •Led in vivo preclinical sample analysis (rodent and NHP), integrating PK/PD and biomarker data to inform translational decisions. •Collaborated cross-functionally, trained teams on bioanalytical workflows, and presented findings to stakeholders and senior leadership to advance siRNA therapeutic programs.

• Generated custom iPSC lines with knock-in, knock-out and point mutations using CRISPR/Cas9 and TARGATT platforms to fulfill client specific research objectives. • Developed and optimized workflows for PBMCs, fibroblasts, and T-cells, including iPSC generation, trilineage differentiation and NPC differentiation. • Performed transfections, FACS sorting, immunocytochemistry, and imaging; cultured, expanded and banked primary mammalian cells, cancer cell lines, and T-cells under aseptic conditions. • Collaborated with cross-functional teams to troubleshoot challenges, streamline workflows, and ensure high-quality project delivery. • Maintained accurate lab records and presented data to senior leadership to support decision-making. • Mentored and trained junior associates, enhancing team expertise in core techniques and contributing to successful project outcomes.

• Designed and delivered Biochemistry and Biotechnology curriculum and lab sessions for over 100 students while key academic goals were achieved. • Worked closely with a multidisciplinary team to boost student performance by 20% through interactive lectures, seminars, and workshops. • Evaluated assignments, projects, and quizzes while providing constructive feedback and progress reports to support student growth. • Mentored and guided students on academic and career paths, helping them to strengthen skills, and prepare for future opportunities.

Stem Cell Culture Lab Course: Conducted experiments to test the human MSCs growth and their differentiation using various assays. Collaborated with teammates to analyze, evaluate and propose experimental plans for future research.