Preeyam Patel, PhD

Driving translational strategy for immune-mediated disease programs through biomarker strategy, data integration, and precision medicine approaches, with oversight of assay development and qualification. Leading cross-functional efforts in target validation, biologics advancement, omics analytics, and clinical translation.

- Oversight, management, and analysis of PanCancer IO 360 Nanostring assay for discovery biomarker studies in phase 2 (NSCLC and HNN) and phase 3 (Melanoma) clinical trials. Intention to identify immune signatures associated with treatment response and add to mechanism of action of our therapeutic. -Investigation and classification of the tumor microenvironment across indications using spatial biology, with particular interested in AI-driven cell calling and identification of cellular neighborhoods -Validation of novel biomarker platforms for spatial transcriptomics such as: GeoMx/Cosmx and Visium/Xenium for incorporation into future biomarker pipelines -Use of Nanostring TCR Diversity assay to assess whether there are changes to TCR Variable regions following healthy human PBMC stimulation with singular peptides. See SITC 2023 poster titled "T Cell Receptor Diversity Analysis of in vitro-Expanded T Cells Against IDO1 and PD-L1-Derived Peptides" here: https://iobiotech.com/wp-content/uploads/2023/11/SITC-Poster-1029-T-cell_Preeyam-Patel.pdf

- Continued working under John Li, former CEO of Salubris Bio - Company Focus: "discovery and global development of first-in-class biological therapeutics for the treatment of cancer, cardiovascular and autoimmune diseases" - Preclinical and translational lead for novel ADCs for autoimmune disease - Coordinate immunological studies across teams (China and US), lead project meetings and discussions - Oversight, management and participation in pre-clinical pipeline development: Novel target discovery using informatics, validation of target using publicly available databases, test functionality of novel antibodies, development of novel cell-based assays for functional validation, design studies to test efficacy, design pre-clinical animal models for testing, discussion of potency assay for FDA, drafting of IND application -Facilities and vendor management and operations

- R&D and translational research for pre-clinical bispecific antibodies in cancer and heart disease - Early target discovery, drug validation, experimental design for MoA and execution of efficacy studies, performance of cytotoxicity assays, planning and testing in pre-clinical animal models, optimization of translational release assays, pre-IND applications

Journal Selection Assistant: -Make 3 to 5 recommendations for journal submission based on manuscript findings, journal expectations, scientific topic, and client requests Peer Review Service -Act as journal reviewer and provide constructive feedback on manuscript contents for clients

- Bernard B. Levine Research Fellow in Immunology - Mentor: Dr. Robert Schneider https://med.nyu.edu/faculty/robert-j-schneider - Projects: Determining i) the role of canonical cap-dependent translation in the development of T follicular helper (TFH) cells and germinal center (GC) B cells in the context of autoimmune multiple sclerosis, ii) effects of non-canonical cap dependent translation on regulatory T cell development, effector function, and colitis. Publication: Patel P, Perez B, Walters B, Orlen M, Volkova A, Ruggles K, Park C, Schneider R. “Translational regulation of TFH cell differentiation and autoimmune pathogenesis” Science Advances 2022 Jan 21 (Vol 8, Issue 25) Link: https://www.science.org/doi/10.1126/sciadv.abo1782

"Can we prevent allergies altogether?" : https://medium.com/@Sparrho/can-we-prevent-allergies-altogether-a448b0dfbdd9 Pinboard: https://www.sparrho.com/pinboard/using-microbes-to-balance-the-immune-system-and-prevent-asthma/167395/?utm_source=briefing_20171020&utm_medium=email&utm_campaign=citation_%3C%3CFirst%20Name%3E%3E%3C%3CLast%20Name%3E%3E

- Mentor: Dr. R. Lee Reinhardt https://www.nationaljewish.org/doctors-departments/r-lee-reinhardt - Projects: Investigate i) the role of AP-1 transcription factor, BATF, in lung and intestinal ILC2 subset function, generation, and migration ii) how neonatal helminth-derived glycans can "feed" the microbiota and promote maturation and seeding of regulatory T cells in the intestine for prevention of allergy Publication: Miller M, Patel P, Bao K, Danhorn T, O’Connor B, Reinhardt, L. “BATF acts as an essential regulator of IL-25-responsive migratory ILC2 cell fate and function.” Science Immunology 2020 Jan 10th (43) Link: https://www.science.org/doi/abs/10.1126/sciimmunol.aay3994

Mentor: John Kearney. Our laboratory’s research interest is the “hygiene hypothesis” and we have investigated mechanisms by which early microbial exposure can dampen the development of allergic disease. The primary finding of my research is that allergens and bacterial species can display conserved bacterial epitopes (glycans and phospholipids). Neonatal exposure to these bacterial species generates antigen-specific B cells and antibodies that target these conserved epitopes on allergens to inhibit engagement of innate-like receptors on dendritic cells in the lung and suppress the generation of TH2 cells and the development of allergic disease during adult life. Publications: Patel, P and Kearney, J.F. 2015. Neonatal Exposure to Pneumococcal Phosphorylcholine Modulates the Development of House Dust Mite Allergy During Adult Life. Journal of Immunology 194 (12) 5838-5850; June 15, 2015 Published ahead of print on May 8, 2015. Patel, P., King, R., and Kearney, J.F. 2016. Pulmonary α-1,3-Glucan-Specific IgA-Secreting B Cells Suppress the Development of Cockroach Allergy. Journal of Immunology 197 (8) 3175-3187; Published ahead of print on August 31, 2016. Patel, P and Kearney, J.F. 2017. CD36 and Platelet-Activating Factor Receptor Promote House Dust Mite Allergy Development. Journal of Immunology 2017 Aug 1st 199 (3) 1184-1195.Published ahead of print on June 30, 2017. Reviews: Kearney, J. F., P. Patel, E. K. Stefanov, and R. G. King. 2015. Natural Antibody Repertoires: Development and Functional Role in Inhibiting Allergic Airway Disease. Annual review of immunology. Patel, P and J. F. Kearney. 2015. Immunological Outcomes of Antibody Binding to Glycans Shared Between Microorganisms and Mammals. Journal of Immunology Brief Review 197 (11) 4201-4209.

Diagnostic Clinical Microbiology, Mycology, and Parasitology

Mentor: Dr. John F. Kearney Committee: Drs Casey Weaver, David Briles, David Chaplin, Chad Steele Dissertation Topic: “Antigen-specific B cell stimulated as a result of neonatal microbial exposure suppress the development of allergic disease during adult life” Research Interests: infectious disease, allergy, humoral and cell-mediated immunity Funding: July 2015-present: Institutional Predoctoral T32 Training Award Recipient

Past Clinical Rotations and Experiences: Duke University Medical Center (Durham, NC): Hematopathology, Coagulation Studies, Molecular Genetics The University of North Carolina at Chapel Hill Hospital (Chapel Hill, NC): Microbiology, Immunology, Hematology, Body Fluids, Blood Bank, Cytology Veterans Affairs Medical Center (Durham, NC): Chemistry Durham Regional Medical Center: Phlebotomy, Transplantation Medicine