Prapti Vyas

• Executed large-scale longitudinal NHP pharmacology and biodistribution studies (25–30 animals, 2–5 months) for a circRNA CAR-T program; developed and optimized high-parameter flow panels and performed high-dimensional data analysis • Evaluated RNA transgene expression from LNP and electroporation delivery in primary human and cynomolgus T cells • Co-authored technical reports for pre-clinical regulatory filings; collaborated cross-functionally to define study endpoints and program strategy • Presented data to program leadership and companywide stakeholders to drive candidate prioritization and experimental direction • Generated large-scale CAR⁺ T cell batches for CRO clinical assay optimization; developed assays to evaluate CAR stability in Cyto-Chex tubes across multiple timepoints

• Designed, executed, and analyzed experiments for an in vivo LNP-delivered RNA CAR-T program targeting oncology and autoimmune indications • Optimized 3 comprehensive flow cytometry panels (antibody titration, FMO controls); performed large-scale longitudinal analysis (FlowJo/Prism) supporting go/no-go decisions • Executed NHP studies for in vivo RNA CAR-T: tissue processing, immunophenotyping, and high-dimensional analysis demonstrating B-cell depletion and CAR expression • Optimized ELISpot and multiplex cytokine profiling assays; served as Attune NxT Super User managing instrument maintenance and troubleshooting • Mentored junior scientists in primary cell isolation, immunostaining, and data acquisition; coordinated cross-functional sample collection logistics

• Optimized MLR and cytokine assays to characterize LILRB2/4 antagonist activity in primary T cells and macrophages • Applied CRISPR-Cas9 for B2M knockout and target validation in THP-1 cell lines • Managed Luminex cytokine assay data within ELNs for cross-functional reporting; directed intern training in cell culture and multiplex assays

Immuno-oncology, CRISPR, Cancer biology, Immunotherapy

• Improved the process of iron staining and MPO (Myeloperoxidase). Performed Test for antibodies • Operated Neo immocor, fully automated and optimize the workload by giving quality test results for forward and reverse blood grouping • CD4 count by Caliber- FACs. Partial thromboplastin time (PT) and Activated partial thromboplastin time (APTT) to examine speed of blood clotting using Acl-top. • Urine examination by UC-3500 and UF-5000 • CBC count using ADVIA 2120 • Other manual test: Fibrin Degradation products, Indirect and direct coombs test, Treponema pallidum hemagglutination assay, Microfilaria test, Malaria parasite by digital cytometry using Sight machine, Blood group using slide and tube method.

Protein Chemistry, Molecular cell Biology, Experimental Design and Bio- statistics, Cell culture process, Introduction to Biotechnology.

The Biotechnology Enterprise, Protein Chemistry, Molecular cell biology, Downstream processing, Cutting Edge application of biotechnology, Biostatistics.