pooja kanani

* Project: To analyse allele frequencies and heterozygosity of factor VIII and factor IX gene polymorphisms in the indigenous population of Nepal * Performed DNA extractions from peripheral blood by traditional phenol-chloroform and kit method * PCR to amplify six different polymorphisms on f8 and f9 genes of X chromosome * Gene-tracking by RFLP – PAGE

* Project: Development of vaccine against African Swine Flu * Cloning of synthetic genes and expression of recombinant proteins in baculovirus and lentivirus * Optimizing process for generation of chimeric proteins * Evaluating protein expression by immunocytochemistry, Dot Blot; Purifying proteins by affinity chromatography; Quantifying proteins by BCA assay * Using aseptic tissue culture techniques to handle mammalian cell lines such as HEK 293A and insect cell lines such as High-Five and Sf-9

* Project: Immunogenicity of a Recombinant Rift Valley Fever MP-12 Vaccine * Evaluating the neutralizing antibody response by Plaque Reduction Neutralization (PRNT80) * Measuring the optimum dose ( 1×101 - 1×107) and most effective of route of administration (intramuscular or subcutaneous) * Quantified IgG antibodies by indirect ELISA to support PRNT80 data

* Market Analyst Intern, Office of Strategic Initiatives, Texas A&M University Systems * Understanding structure and function of Center for Innovation in Advanced Development and Manufacturing (CIADM) * Conducted thorough research on anthrax and influenza vaccine technologies in phase trials and company pipelines * Extensive research of BARDA, NIH biodefense projects and investments to identify potential task orders for CIADM

* cGMP compliant testing of in process samples and final products * HPLC, Agarose gel electrophoresis, Nucleic acid quantification, Phage assay * Bioburden, pH and conductivity, environmental monitoring * Developed, reviewed, edited SOPs * CAPA and deviation reports

WHARF Youth(Wockhardt- Harvard Aids Research Foundation), organized and conducted 7 events all over Mumbai with an objective to spread AIDS awareness. - Sports event held by Dynamics Annual Sports event conducted by D. Y. Patil Sports Academy (Inter College Competition) Activities and Societies: -Volunteer @ Wharf Youth -Sports: 2nd position in Badminton

Activities and Societies: Historian for the Biotechnology Society