Patrick Brehio

Operational and project management support for the Preclinical Development group. - Manage contract initiation and finalization, collaborating with internal and external stakeholders to draft, edit, and process agreements and purchase orders. - Track preclinical study progress, manage data and reports in internal systems, and ensure accurate submission of finalized study reports to regulatory agencies.

Engineer structural protein variants to enhance gene editing efficiency, leveraging high-throughput screening and next-generation sequencing methods. - Develop and optimize workflows.

Developed, optimized, and executed high-throughput antibody screening assays supporting multiple discovery and clinical programs. - Gained hands-on experience in quality management systems, lot review, and change controls through an internal Quality Assurance job shadowing program. - Engineered stable cell lines and developed high-throughput screening assays (ELISA, qPCR, reporter genes) to support discovery efforts and clinical candidates SRK-015 & SRK-181.

Designed and executed cell-based assays to support several early discovery stage programs. - Optimized cell-based assays using Luminex and flow cytometry. - Positive and negative selection of lymphocyte populations from peripheral mononuclear blood cells.

Managed lab operations and assisted with sequencing and genotyping projects for Ebola and Lassa virus research. - Led lab operations as assistant lab manager, optimizing workflows, implementing Six Sigma techniques, and enhancing sample tracking with a laboratory information management system.

Prepared DNA samples for exome sequencing using established library construction and hybrid selection procedures. - Oversaw quality control and continuous improvement of independent or collaborative development projects.

Purified and analyzed proteins using HPLC. Managed lab inventory and prepared peptide samples for shipping. - Ordered and tracked inventory of lab materials used by the peptide purification group. - Maintained communication between the peptide synthesis and purification labs to improve efficiency.