parvinder kaur

senior executive scientist

About

I have done MTech Biotech with 8 year experience in Molecular and cell Biology. Working as Sr. Executive scientist. Expertise in Bacterial, Mammalian and Bacculovirus expression system for the development of recombinant proteins. Cloning, expression and purification of Diagnostic Markers including cancer markers like rSHBG, rTPO, rCD279, MUC10, MUC20 tandem repeats (15-3 like antigen) antigen in mammalian expression system. Cloning, expression and purification of Diagnostic Markers in bacterial expression system

Country

india

City

bombay

Industry

biotechnology

Skill

molecular biology, biotechnology, cell biology, drug discovery, genomics, elisa, pcr, cell culture, purification, protein purification, lifesciences, sds page, life sciences, cro, dna, microbiology, polymerase chain reaction, yeast, protein chemistry, bioinformatics, recombinant dna, biochemistry, validation, cancer, western blotting, chromatography

Experience

senior research asssociate

gvk biosciences

2012-4 - 2013-3 · 1 yr

I am working in Molecular and Cell biology. cloning and expression of recombinant proteins in Bacteria, yeast, Bacculovirus and mammalian system. Site directed mutagensis. PCR based cloning.

research associate

premas biotech

2009-7 - 2012-3 · 2 yrs 9 mos

Working in molecular biology lab. cloning and expression of recombinant proteins in Bacteria, yeast and mammalian system. Site directed mutagensis. PCR based cloning.

senior executive scientist

yashraj biotechnology ltd

2013-5 - Present · 13 yrs 5 mos

I have establishment of Mammalian and Bacculovirus expression system for the development of recombinant proteins. Cloning, expression and purification of Diagnostic Markers including cancer markers like rSHBG, rTPO, rCD279, MUC10, MUC20 tandem repeats (15-3 like antigen) antigen in mammalian expression system. Adopted CHOK1 and AD293 cells in serum free medium and stable cell line generated of rSHBG, rTPO, rCD279, MUC10, MUC20 tandem repeats (15-3 like antigen) antigen, purified all protein and validation done for production. HuH7 cell line adopted in serum free medium for AFP and AFP-L3 secretion. Development of Cell derived proteins and Media optimization for High yield expression. Maintenance of mammalian cell line (like CA-19-9, CA15-3, CA125, HBA1c Myeloma cell Line antibody and Standard cell lines Cloning, expression and purification of Diagnostic Markers in bacterial expression system