Paisley Haskell

Screened hundreds of novel CAR constructs in collaboration with the Drug Discovery team and contributed to a crucial drug candidate selection decision in major company-wide in vitro program research. | Trained and led internal circular RNA (oRNA) sequencing team and validated thousands of oRNA products crucial for internal and external production and research purposes. | Designed and executed high-throughput pooled screening methods to identify top performing constructs. | Active member of ELN Steering Committee, Community Outreach team, and Platform meeting committee

Evaluated novel designs and applications of oRNA as in vitro project lead. | Collaborated with the Immunology team to screen hundreds of CAR constructs using molecular biology techniques. Played a key role in the selection of a critical drug candidate during a major company-wide in vitro research program. | Studied in vivo biodistribution and stability over time of oRNAs delivered via LNP dosing to mouse tissues for genetic disease program, by tissue homogenization, RNA isolation, and qPCR.

Designed and evaluated novel strategies for CRISPR-Cas9 gene editing and regulation via AAV delivery in vitro and in vivo. | Developed and optimized various molecular biology assays for gene editing detection and protein expression. | Conducted cross-functional projects that integrated CRISPR technology with complex gene regulatory mechanisms.

Processed tissue samples for formalin-fixation, embedded tissues in paraffin (FFPE), and sectioned and stained FFPE and frozen tissues as requested by various MGH research labs. | Performed standard histological staining methods including hematoxylin and eosin, immunohistochemical (IHC), and more.

Compared the in vitro efficiency of editing and gene expression of various Cas9 gene circuits delivered via AAV. | Generated target vectors by restriction enzyme cloning and delivered to primary mammalian cell lines by AAV transduction. | Measured editing efficiency and gene expression by amplicon sequencing and qPCR.