natalia szydlowska

establishing of reliable way to combine the variable regions of human T- cell receptors (TCR) with the mice constant regions with the long range goal to facilitate a rapid generation of TCR transgenic mice and analysis of key events in Diabetes type II analysis of blood samples from patients suffering from Diabetes type II and children with high risk factor via single cell PCR in order to establish a baseline for development of reliable tool for Diabetes diagnostic.

* making 3D structures using microlithographic techniques * synthesis, purification and characterization of organic compounds and peptide conjugates using preparative and analytical methods including analytical and preparative HPLC, MALDI-TOF-MS, 1H- and 13C NMR * synthesis and characterization of sensoric polymerizable diacetylene oligopeptide gels

* optimization of protein isolation and purification protocols * extraction, isolation and purification of tubulin proteins from different Chlamydomonas strains (wild type and mutated strains) via ion exchange chromatography * performing of tubulin polymerization using two different protocols * imaging of polymerized tubulin (microtubules) using Total Inverted Reflection Fluorescence Microscope * SDS- PAGE of purified tubulin proteins using commercialy available gels * casting of big-sized acrylamide-Urea gels for resolving of different dynein protein chains * 2D gel electrophoresis including rehydration, isoelectric focusing and SDS- PAGE followed by Coomassie staining * silver staining