Namrata Menon

- Developed and optimized flow cytometry- and qPCR-based cell assays for oligonucleotide screening and target knockdown evaluation to support platform discovery programs and enable Go/No-Go candidate selection decisions. - Designed and implemented a flow cytometry-based antibody epitope binning assay to complement CRO-led antibody discovery campaigns, accelerating development candidate (DC) selection timelines by approximately 2 months. - Executed RNA isolation, NGS library preparation, and Illumina sequencing workflows for mouse tissue samples from in vivo preclinical studies, supporting transcriptomic and gene expression analyses. - Collaborated cross-functionally with discovery biology, platform, and automation teams on experimental design, assay optimization, troubleshooting, data interpretation, and workflow improvements in a fast-paced R&D environment.

- Spearheaded as a lab lead for radio-ADC program from proof of concept to DC, managing experimental design and high-throughput screening strategies, which helped meet project milestones and informed key program decisions. - Designed and executed crucial pre-IND studies, co-authoring IND documentation, contributing to regulatory strategies, and aiding in the progression toward first-in-human submissions. - Developed and optimized high-throughput cell based and functional assays, such as flow cytometry assays, ELISA, Western Blot, Incucyte and Octet assays to effectively support hit identification and lead optimization efforts. - Drove automation innovation by implementing the Hamilton liquid handler and creating custom VENUS methods for normalization, hit picking, and ELISA, significantly boosting assay reproducibility and throughput. - Collaborated with multidisciplinary teams, including pharmacology, chemistry, radiochemistry, tech ops, to integrate scientific insights, which aligned candidate selection strategies across the board. - Presented scientific findings, including proof-of-concept studies and mechanistic data, to project teams and leadership, supporting strategic decisions and prioritizing program goals. - Provided technical leadership and mentorship, onboarding new scientists and research associates, which improved team capabilities in assay development, automation, and data analysis.

- Led the screening workflow for programs acquired from TeneoBio, successfully implementing new protocols that increased sample handling capacity by 200%, which streamlined operations and improved throughput. - Developed assays using iQue flow cytometry with various labeled beads to identify a range of antibody epitopes, helping categorize them into different bins to better understand panel diversity and ensuring inclusivity in subsequent assays. - Performed 384-well CX7 binding assays to assess the binding capabilities of antibodies, which enabled the initial selection of high binders targeting specific antigens expressed on 293T transfected cells and endogenous cell lines, thereby optimizing the screening process.

- Collaborated as an SME for bioanalytical assay development, optimization and automation of gene therapy programs to analyze and detect efficient clones and also product-related impurities. - Spearheaded functional team for assay development of priority projects and executing delivery in 50% of the expected turnaround time and was in charge of the experimental design, statistical analysis, qualification, and documentation. - Developed a cell-based assay for a protein knock down quantification using MSD technology. - Evaluated different Cell quantification/imagining platform such as CX5, Celigo and Operetta for optimal quantification and analysis of functional cell-based assay. - Performed HT AAV genome titer assays to obtain genome copy numbers for dosing purposes, supporting the Gene Therapy Programs in 96 and 384 well formats. - Proficient in using ddPCR, handling the Bio-rad Automated Droplet Generator along with the Qx200 Droplet reader and analyzing data with Quantasoft Software. - Implemented newer version of assay protocol that used newer technologies to reduce the volume of the costly critical reagents used by 60% and cutting assay time short by 50%. - Conducted independent studies of critical reagents, showing the shelf life was 6X more than advertised by seller, saving the company around $50,000 per year - Partnered with PD team and manufacturing teams to cater to on-going assay development demands with a goal to establish clinically and commercially viable product - Maintained, operated and troubleshooted automated systems such as Hamilton Microlab Star and Vantage Liquid Handling system and collaborated with automation team to make existing assays high throughput. - Maintained a clean and organized inventory of the lab consumables and collaborated with the KanBan lab services to replenish the stock and promptly replace out of stock items with alternatives. - Proficient in the use of LIMS Software, MyCIMS and ELE for result submission and documentation.

• Performed HT primary and secondary screening and characterization of transfection supernatants/purified antibodies, EC50, IC50, 2-point binding ELISA for determining target binding and potency for early drug antibody discovery • Developed and implemented manual and automated biochemical binding, expression and functional assay using the Liquid handler systems (Caliper Life sciences- Sciclone ALH3000) and the Maestro software • Conducted extensive assay data analysis using MS Excel, Genedata assay analyzer and Graph Pad Prism Pro • Prepared buffers/reagents for ELISA experiments while working in a BSL2 laboratory • Performed re-array, normalization and distribution of purified protein samples using the TECAN EVO100 robot and QC using NanoDrop 8000. Updated the SOP for customer-designed normalization of samples/supernatants • Performed through maintenance of the automation system and developed proficiency in troubleshoot hardware issues

Performed high-throughput primary and secondary screening, characterizing transfection supernatants and purified antibodies to determine EC50, IC50, and binding affinity values in early antibody discovery. Developed and implemented manual and automated biochemical binding assays using the Caliper Life Sciences Sciclone ALH3000 liquid handler and Maestro software, streamlining processes and increasing efficiency.

• Troubleshoot and solve issues associated with hardware, software and OS on both Mac and PC. • Computer support includes hardware diagnosis and remediation, virus/malware removal and both warranty and out-of-warranty computer repair for faculty and students

• Assisted research scientist by maintaining and passaging several cell lines (HeLa, HSF, and immune system cells) • Proficient in the handling and fabrication of microfluidic device with the help of lithographic technique • Collaborated with research and development team to perform single- cell analysis of Natural killer cells including viability, tracking and cell lysis studies against multiple myeloma cells.

• Analyzed hundreds of endogenous metabolites from S.cerevisiae extract using the triple quadruple LC-MS instruments in an inter-disciplinary research lab at the Faculty of Molecular Genetics in collaboration with Agilent Technologies. • Targeted metabolomics experiments conducted focusing on quantifying specific metabolites and large number of samples were used to accurately measure the abundance of a known metabolite. • Developed proficiency in curating multi-compound batch results from MassHunter Quantitative Analysis software • Efficiently interpreted the data collected and understood the biological context of the compounds detected.

• Conducted focused literature searches for carious ongoing cell-biology based research conducted in Harrison Lab affiliated with Canadian Space Agency (CSA) and NASA. • Proficient in selective article retrieval from NCBI, Science Direct, Cell, Nature, etc.; along with database creation, data cleansing, statistical analysis, dissemination of findings and maintaining project integrity • Developed scientific report writing skills with effective visualizing large data sets using graphic tools.