Mohanraj Gopalswamy

Ø  Lead biophysicist responsible for NMR, MST, nanoDSF, ITC, DSC, SPR, CD, DLS/SLS, and SEC-MALS instrumentation across active drug discovery programs Ø  Teaching and curriculum development for Quality Control and Validation of Pharmaceutical Products in the Master of Industrial Pharmacy program Ø  Biophysical characterization of recombinant proteins and protein–protein complexes for oncology and therapeutic research programs Ø  Supported structure-based drug discovery through high-quality protein production and biophysical data analysis Ø  Contributed key data for US and WO patent filings supporting first-in-class RUNX1/ETO small-molecule inhibitor development Ø  First-author publications in Scientific Reports (2022, 2025) focused on anti-leukemic drug candidates and NHR2 interface targeting Ø  Generation, interpretation, and presentation of scientific data in multidisciplinary and cross-functional collaborations Ø  Coordination of scientific projects and data exchange with academic and industrial collaborators Ø  Supervision, mentoring, and technical training of PhD students, Master’s students, technicians, and visiting scientists Ø  Independent leadership of projects involving proteins, peptides, GPCRs, DNA/RNA, fibrils, polymers, nanoplastics, and multifunctional complexes Ø  Selected for the Academic Career Development Programme at HHU Düsseldorf (Top 10 candidates)

Ø  Expansion of business opportunities across Eastern Europe, Finland, and the Middle East Ø  Customer relationship management, prospect qualification, and account expansion Ø  Sales, technical support, and scientific consultation for the complete NanoTemper portfolio Ø  Performance of Proof of Concept (POC) studies and instrument demonstrations Ø  Development and execution of strategic business development initiatives Ø  Delivery of on-site installations, customer training, and scientific enablement programs Ø  Cross-functional collaboration with regional sales teams to support account conversion Ø  Representation of the company at international scientific conferences and customer sites ------------------------------------------------------------------------------------------------------------------------- ▪ Territory & Business Development Managed the full commercial cycle across Eastern Europe, Finland, and the Middle East, including pre-sales consultations, instrument demonstrations, customer adoption, and account expansion. ▪ Portfolio & Product Expertise Supported NanoTemper’s biophysical platforms including Monolith (MST/MOX), Prometheus Panta (nanoDSF), Dianthus, and Andromeda, aligning technical capabilities with customer workflows in drug discovery and protein characterization. ▪ Customer Success & Training Delivered on-site installations, workflow optimization, and customized user training covering binding affinity, protein stability, and affinity screening applications for pharmaceutical and academic customers. ▪ Cross-functional Collaboration Partnered with regional sales teams to qualify opportunities, address scientific challenges, and position NanoTemper solutions effectively against competing technologies. ▪ Brand Representation Represented NanoTemper at international conferences, exhibitions, and customer meetings, strengthening relationships with key stakeholders across multinational markets.

Ø  Lead biophysicist and NMR scientist supporting active drug discovery pipelines in oncology and CNS/neurological research programs Ø  Expertise in MST, nanoDSF, ITC, DSC, SPR, NMR, CD, DLS/SLS, and SEC-MALS instrumentation for protein and ligand characterization Ø  Design and execution of NMR strategies including STD-NMR fragment screening, HSQC/CSP binding-site mapping, affinity, kinetics, and mechanism-of-action studies Ø  Biophysical characterization of recombinant proteins, GPCRs, peptides, protein–protein complexes, DNA/RNA, fibrils, polymers, and nanomaterials Ø  Expression and purification of isotopically labelled proteins (15N, 13C, 2H, 19F) for advanced protein- and ligand-observed NMR applications Ø  Operation and maintenance of multi-frequency Bruker NMR spectrometers (400 MHz–1.2 GHz), including cryogenic probe handling and routine maintenance Ø  Supported structure-based drug discovery through integrated biophysical and structural biology workflows Ø  Contributed key biophysical and NMR data supporting US and WO patent filings for first-in-class RUNX1/ETO anti-leukemic inhibitors Ø  First-author publications in Scientific Reports (2022, 2025) involving 15 complementary biophysical techniques and NHR2 interface targeting Ø  Generation, analysis, interpretation, and presentation of scientific data in multidisciplinary and cross-functional collaborations Ø  Coordination and supervision of collaborations with CROs, academic institutions, and industrial partners

Ø  Lead NMR scientist supporting biophysical characterization of recombinant Membrane proteins, IDPs, peptides, protein–protein complexes, RNA, fibrils, polymers, and nanomaterial Ø  Expertise in ITC, NMR, CD, SAXS, DLS/SLS, and SEC instrumentation for protein and ligand characterization Ø  Expression and purification of isotopically labelled proteins (15N, 13C, 2H, 19F) for advanced protein- and ligand-observed NMR applications, sortase mediated ligation for larger complexes Ø  Operation and maintenance of multi-frequency Bruker NMR spectrometers (400 MHz–1.2 GHz), including cryogenic probe handling and routine maintenance Ø  Supported structure-based drug discovery through integrated biophysical and structural biology workflows

Ø  PhD Biophysicist and Structural Biologist specializing in Class B GPCRs and peptide hormone signaling, post-translational modifications, protein aggregation and amyloid systems Ø  Recombinant protein expression (mini-fermenter), purification (HPLC, SEC, IEX, affinity), inclusion-body refolding, IDP and membrane-protein reconstitution (micelles, GUV/SUV liposomes, nanodiscs, bicelles) Ø  Research expertise in the Parathyroid Hormone (PTH) system (PTH1R/PTH2R) related to osteoporosis and hypoparathyroidism Ø  Author of peer-reviewed publications in PNAS, BBA, and ACS Chemical Biology, etc. Ø  Application of high-resolution biophysical techniques, protein engineering, and cell-based assays to study receptor activation mechanisms Ø  Investigation of residue-specific interactions and conformational dynamics governing ligand–receptor function Ø  Experimental validation of GPCR binding models and characterization of post-translational modifications Ø  Research on hormone aggregation, amyloid behavior, and peptide stability Ø  Contribution of structural and mechanistic insights supporting drug discovery and development for metabolic and endocrine diseases ------------------------------------------------------------------------------------------------------ Core Expertise • Structural Biology & Biophysics: Solution NMR (including HSQC, TROSY), SPR, ITC, CD, FTIR • Protein Engineering & Biochemistry: Recombinant expression (E. coli), advanced purification (FPLC/HPLC), membrane protein refolding, SUMO-fusion systems • Recombinant protein expression , purification (HPLC, SEC, IEX, affinity), inclusion-body refolding, IDP and membrane-protein reconstitution (micelles, GUV/SUV liposomes, nanodiscs, bicelles) • Analytical & Functional Assays: Mass spectrometry (MALDI-TOF, ESI-MS/MS), TEM, cAMP and kinase assays in HEK293 cells • Research Focus: GPCR, ligand–receptor dynamics, post-translational modifications, protein aggregation and amyloid systems

________________________________________ NMR Research Centre, IISc Bangalore — India's #1 ranked research university ________________________________________ • Ran 1D & 2D NMR experiments (COSY, HSQC, HMBC, NOESY) for pharma companies and research institutes across India • Characterized small organic molecules using heteronuclear NMR, supporting analytical and R&D teams in structure elucidation • Managed CryoProbe operation and cryogen fills (LN₂ & LHe), maintaining instrument reliability in a high-throughput facility • Assisted in NMR spectrometer installations and commissioning, including shimming, system qualification, and hardware setup • Trained graduate students in NMR instrumentation, data acquisition, and experimental best practices ________________________________________ Key Skills: NMR Spectroscopy · 1D/2D NMR · COSY · HSQC · HMBC · NOESY · DEPT · CryoProbe · Cryogen Handling · Small Molecule Characterization · Structure Elucidation · Bruker NMR · Scientific Training ________________________________________

• Involved in designing and development of various ligand centric databases (from Patents) of Protease and GPCRs for drug discovery projects. • ISIS draw and base 2.5 versions, Chemoffice version 9, ACD labs software, are the software tools used.