Min Xie

We are doing Pre-clinical research of TCE and ADC.

Listeria-based vaccines are highly promising vectors for cancer immunotherapy. The goal of this NIH-funded project was to develop a novel Listeria-based vaccine targeting CD105 to treat Renal cell carcinoma (RCC). CD105 is strongly expressed on proliferating endothelial cells such as those in tumor vasculature but, also on RCC tumor cells themselves. Therefore, targeting CD105 is a promising strategy for RCC cancer treatment. Our Listeria-based CD105 vaccines infect dendritic cells (DCs) that will process and present CD105 peptides on MHC class I and II molecules. These mature DCs will then activate CD105-specific CTLs that target both tumor cells and tumor-associated vasculature on RCC tumor models. My responsibilities • Culture primary immune cells (T cells and dendritic cells), tumor cells lines (human and murine), and bacteria (E. coli and L. monocytogenes). • RCC tumor models (subcutaneous and orthotropic) • Flow cytometry including staining cells, operating BD Fortessa, developing compensation panels, and data analysis • Design and conduct immune-related experiments, like ELISA (serum cytokines), IHC, Western blot, etc. • Manage large animal colony including knockout and transgenic mice and collect tissues from animals to perform genotyping (ear punches, blood samples, tail snips) • DNA and RNA purification for transfection, sequencing, and quantitative PCR • Conduct animal experiments under IACUC-approved protocols using Listeria-based vaccines, antibody-based receptor blockade, etc. • CD8+ T cells, CD4+ T cells, naïve CD4+ T cells sorting by magnetic-bead separation • Train new students 项目1介绍：肾细胞癌（RCC）是美国肾癌最常见的种类，发病率并逐年攀升。CD105是一种特异分子大量表达于RCC的癌细胞和癌组织附近脉管系统中，李斯特菌疫苗作为一种优秀的载体可以特异感染树突状细胞并诱导产生对CD105特异的CD4和CD8阳性T细胞，从而双重追踪和杀灭癌细胞和肿瘤相关血管上皮细胞。 工作职责：1通过体外细胞实验探索感染过程的细胞信号通路以及相关细胞因子的参与过程；2通过动物实验验证构建的减毒李斯特菌疫苗的活性和毒性；3通过免疫类试验检测细胞因子在感染过程的差异；4实验室管理，试剂购买以及实验室统筹技术和质量控制。

small molecule inhibitors target cancer related genes

Adoptive cell transfer (ACT) is the transfer of cells into a patient to treat disease. The cells used in ACT originate either from the patient or another individual. The cells are most commonly derived from the immune system with the goal of improving immune functionality and characteristics. In autologous cancer immunotherapy, T cells are extracted from the patient, genetically modified to target the cancer cells, cultured in vitro, and returned to the same patient. My responsibilities • Conduct adoptive cell therapies for cancer patients, such as CTL or DC-CTL, NK, etc. • Separate PBMC from patients' blood samples by density gradient centrifugation, culture and differentiate T lymphocytes or DCs into cytotoxic T lymphocytes or activated DCs • Assess patient tumor sensitivity to various drugs to guide patient treatment strategy • Assist in new drug clinical trials such as assessing drug metabolism in blood samples • Purchase and maintain lab instruments • Develop laboratory standard operating procedures and maintain compliance • Train new students and workers 项目1介绍：免疫细胞过继疗法主要是从患者外周血抽取一定量的免疫细胞进行体外培养和诱导分化，例如细胞毒T细胞，NK细胞，树突状细胞等，通过一定的周期培养后，进行放大培养，细胞类型的鉴定和亚群分析，同时进行细菌内毒素，活菌落，免疫细胞数量计数等质控手段将合格的细胞制品回输给患者体内。 工作职责：1负责日常细胞培养，制定实验室规章，质量控制，2实验室环境卫生标准检测，实验室灭菌，实验物资的购买，员工培养和考核，3新实验室的项目对接，验收，实验室仪器的维护保养购买，4患者肿瘤组织的药物敏感性筛选，新药临床的样本采集和处理，5研究生毕业课题培养，以及实验室统筹管理工作。 项目2介绍：大鼠放射性肺炎的药物干预，本项目主要研究放射治疗对癌症患者正常肺组织的影响以及各种药物治疗的有效性对比，指标主要进行血清炎症因子的检测（ELISA）和肺组织的IHC免疫组化的对比。 工作职责：1监督整个实验的样本采集，细胞因子的检测，2动物实验的质量控制和流程制定，数据采集和生物学统计。

Project introduction Chongqing Fa Gen Biological Pharmaceutical developed novel therapeutics for a multitude of disease states including cancer, diabetes, and tissue fibrosis. My responsibilities • Purify recombinant human-canine chimeric uricase by ammonium sulfate precipitation, ion-exchange resin, and molecular sieve chromatography • Culture cells and conduct candidate drug bio-activity test in vitro by MTT, MTS or CCK-8 • Perform HPLC analysis, SDS-PAGE and DNA agarose gels • Research on animal disease models such as pulmonary fibrosis (rat), cornea fibrosis (rabbit), and liver fibrosis (rat) • Conduct experiments on uricase kinetics of enzyme-catalyzed reactions • Familiar with GMP lab and sterile working conditions • Assist in training of new students 项目介绍: KGF突变体抗纤维化的研究。本项目属于国家级重大专项（2012zx09103301-052），主要是人工体外将天然的KGF因子进行二硫键重组，体内试验证明此突变体对各种纤维化例如博来霉素造成的肺纤维化，四氯化碳造成的肝纤维化和乙醇造成的角膜纤维化有抑制作用。 工作职责：1负责突变蛋白质的纯化和HPLC质量控制，体内体外活性的测定，急性毒性和长期毒性的分析，2参与公司的其他三个项目的活性测定, 人犬嵌合的尿酸酶活性以及酶促动力学活性测定以及制剂稳定性考察，水蛭素的体外活性测定，甘精胰岛素的体内活性测定；3辅助其他项目进行。

I studied here for my second Master degree in science and doing research on listeria-based vaccines at Dr. Laurence Wood's lab, we took the same core classes with Ph.D. students.