Mengran Qian

• Explore the role of mis-match repair deficiency in immune checkpoint blockade therapy against glioblastoma. Induced primary glioblastoma in mice model using RCAS based tumor oncogene over-expression in mouse subventricular zone. Knock down the mis-match repair gene in glioblastoma in vivo using shRNA.Established the multi-color FACS analysis platform in our lab and characterized the brain tumor infiltrating lymphocytes especially the infiltrating T cells. Established the PD1 therapy in our mice model and compaired the infiltrating lymphocytes before and after treatment. • Dissection of necroptosis signaling in glioblastoma. Established the inducible necroptosis gene over expression in vivo using CRE-LOXP technique.Established the inducible necroptosis gene knock out in vivo using CRISPR-CAS9 technique. Manipunated necroptosis genes in glioblasoma in vivo, compaired survival difference and pathology readouts. • Investigation function of Proteasome inhibitor in glioblastoma tumor stem cells. Established the proteasome inhibitor therapy in our mice model and compaired survival difference , pathology readouts and Tlx stem cell difference.

• Characterization of human monoclonal neutralizing antibodies against influenza H5N1 virus.
Characterized the neutralizing breadth and potency of human monoclonal antibody 100F4 in vitro and in vivo. 
Mapped 100F4 neutralizing epitope.
Generated hemagglutinin (HA) expression stable cell lines , expressed and purified influenza HA proteins in CHO cells and measured the affinity of antibody 100F4 and 65C6 to wildtype HA proteins and HA from 100F4 escape mutants. Engineered the variable region of our IgG antibody to IgA form and compared the affinity and neutralizing activities. • Neutralizing mechanism of human monoclonal antibody 65C6 and 100F4. Engineered the IgG antibody into ScFv form to exclude the influence of Steric hindrance. Dissected and studied influenza virus infection in three steps: receptor binding, post attachment and fusion . Found that both100F4 and 65C6 can inhibit virus infection through the blockage of low pH trigger virus fusion however 65C6 can also partially inhibit virus binding to its receptor but 100F4 can not. • Mouse T and B cells immune response analysis after immunization and antibody secreting cells sorting for single cell RT-PCR .
Analyzed T and B cell immune response and antigen specific immune response dynamics after mice immunization by FACS and ELISPOT.
Sorted the antibody secreting plasma cells for single cell RT-PCR.