Megan Murt

● Lead research coordinator on 7 cell therapy clinical trials for the multiple myeloma clinical team. Types of trials include Phase I, Phase II, and Phase III Randomized trials. ● Manage patient enrollment and eligibility, study visits, research collections, and study-required testing for 30+ multiple myeloma cell therapy trial patients. ● Generate high-quality documentation that helps ensure protocol adherence across Outpatient and Inpatient clinical teams. ● Ensure patient safety by closely tracking and monitoring patient status and generating and submitting safety reports, as needed. ● Closely coordinate with multiple teams to make sure patient needs are addressed, while maintaining study compliance. ● Maintain accurate and complete study records, including signed informed consent, source documentation, CRFs, drug dispensing logs, and study communications. ● Help lead site initiation visit and study monitor meetings. Address concerns, questions, and requests from study sponsors. ● Routinely update team of safety events and patient status and serve as the point-of-contact for cell therapy trial patients for the multiple myeloma clinical team.

● Coordinated and managed the start-up of a brand-new cell therapy research lab. Brought lab to fully operational in 4 weeks. ● Developed and wrote research and operational protocols and SOPs and established quality efforts around data and electronic documentation. ● Lead developer of multiple functional and phenotyping assays and protocols that were critical for showing proof-of-concept of cTRL’s technology. ● Functional and project lead on multiple projects and workstreams, including Product Cytolytic Activity, Product Potency, TIL Benchmark, Primary Cell Line Generation, and PDX in vivo Model projects. ● Managed and led analysis of large data sets of patient data from multiple workstreams (Research, Engineering, Sample Processing) and contributed to summaries and presentations for senior leadership and board/advisor meetings. ● Received, processed, and analyzed clinical samples from cancer patients, including blood and primary tumors and captured data from patient pathology reports.

Part of the Discovery and Development Team (former T cell Engineering and Biology Team), where I helped push the therapeutic pipeline of pre-clinical research and development of novel TCR and CAR T cell therapies. I specifically worked on a pre-clinical project in collaboration with GSK, helping with the development and pre-clinical data package of a NY-ESO-1 TCR T cell therapy in combination with a T cell anti-exhaustion technology. ● Helped manage and generate the in-vitro preclinical data package for IND submission and patent generation for a next generation NY ESO-1 TCR cell product with enhanced potency. ● Contributed to the pre-clinical data package for a ROR1 CAR-T cell product currently in clinical trials (NCT05274451). ● Managed and led analysis of large data sets while coordinating patient data from multiple assays, including T-cell phenotyping, functional, and safety assays. ● Project lead of assay and protocol development for Seahorse platform for R & D team. ● Wrote and delivered in-depth and detailed pre-clinical research reports and electronic notebook entries for transfer to an outside collaborator to support IND filing. ● Routinely presented research and data at internal and external meetings. ● Helped develop and routinely performed 20+ marker flow cytometry panels for T cell phenotyping and characterization of TCR T cell products on Cytek Spectral Flow Cytometry machines. ● Helped design and execute Incucyte killing assays and other in vitro T cell functional assays, including spheroid, serial re-stimulation, proliferation, and long term growth assays. ● Performed multiplex cytokine assays and analysis for T cell characterization. ● Designed and executed MOA studies (flow and killing assay based) ● Analyzed large data sets and compiled results following high quality standards.

Member of Dr. Philip Greenberg's laboratory (Clinical Research Division, Program in Immunology) where I helped develop novel TCR T-cell therapies for various cancers, including leukemia, pancreatic, and ovarian cancer. I specifically worked on a project screening, developing and characterizing new T-cell receptors that recognize multiple cancer-related antigens and mutations, including WT1, Cyclin-A1, and KRAS. I also helped develop these TCR therapies for multiple HLA types, allowing these kinds of therapies to reach a broader set of the population. ● Stimulated and screened donor CD8+ T cells for endogenous immune responses to cancer antigens- specifically TCR discovery focused on WT1, Cyclin-A1, and KRAS peptides in multiple HLA types. ● Performed functional testing of specific TCRs in primary CD8+ T cells, including cytokine release assays, tumor cell line responses, and killing assays. ● Generated specific TCR T cells for safety experiments in mice and performed immunoassays of mouse blood and tissues to investigate T cell behavior in healthy mice in preparation for future human trials. ● Optimized qPCR analysis of DNA expression of cancer antigens in tumor cell lines. ● Compiled and analyzed large data sets of cellular functional data. Techniques: ● Human and mammalian cell and tissue culture ● Retroviral and lentiviral tranductions into human and mammalian cells ● Human and mammalian immunological cell assays, including Interferon-gamma screening and staining. ● Flow cytometry ● Cell sorting ● Human blood sample processing (Leukapheresis, HLA-typing) ● T-cell activation and characterization, including killing assays ● qPCR with patient samples ● Molecular design and cloning with BioXp, large scale screening/sequencing for correct clones ● Performed data analysis on large, complex data sets

Part of the Protein Engineering Team and the effort to develop and design high performing DNA polymerases. ● Designed novel DNA polymerases and optimized their expression and purification from E.coli. ● Worked to push DNA polymerase function; designed polymerases that would incorporate and extend unconventional nucleotides not found in nature via computational design (Pymol), library design, and RNA/DNA Display technologies. ● Work led to two patents. ● Leader of a two-member team on a polymerase design project. ● Routinely presented data and updates to leadership to help guide decision-making. Techniques: ● Expressing and purifying fusion proteins using E.coli and affinity purification. ● Developing novel Display protocols involving IVTT and protein fusion capture on bead. ● Designing and executing polymerase extension assays using novel nucleotide substrates. ● Library Screening ● Emulsion Formulations ● Display Technology Development ● Protein Conjugations (NHS, maleimide) ● Click Chemistry ● Iterative Computational Protein Design ● Protein Expression and Purification Optimization ● Analyzed large molecular/protein sequence and functional data sets

Member of the Dr. James Priess lab where I investigated molecular and cellular events that occurred during apoptosis in C. elegans- specifically the role of mitochondria and microtubules during cell death. ● Routinely optimized and performed antibody immunostaining and live imaging of C. elegans gonads. ● Maintained an extensive stock of C. elegans strains and set-up new strain crosses as needed. ● Managed lab ordering and inventory.

First member of Dr. Ann Miller’s lab where we study RhoGTPase regulation of cell division using Xenopus laevis. My project utilized live and fixed cell imaging, protein interaction assays, and microtubule manipulations to study Centralspindlin localization at the equatorial cell cortex. I managed all aspects of the lab operations including maintaining lab inventory and supply orders, undergraduate and graduate training and guidance, laboratory safety compliance, and animal care (and have been known to clean and thaw out a freezer a time or two). In addition, I had various research projects where I piloted new techniques for the lab and uncovered novel molecular mechanisms involving cell division. Accomplishments: ● In December 2013, presented poster “Xenopus MgcRacGAP is stabilized at the plus tips of equatorial astral microtubules via an EB1 binding motif” at American Society of Cell Biologists Meeting in New Orleans, LA, which showed how Centralspindlin may be able to localize at the cleavage furrow via microtubules. ● Pioneered various unpublished techniques including monopolar spindle generation in an intact cell epithelium and GST pulldowns with X. laevis embryos. ● Gave research talk for University of Michigan Cytoskeleton Group in June 2013. ● Helped recruit and train four graduate students for PI’s first independent lab. ● Setup lab ordering system and records and wrote various SOPs during lab startup. Adept in: microinjection, SDS-PAGE protein analysis, protein immunoblots, fluorescence imaging including confocal imaging of live cells, mRNA synthesis, protein purification, in vitro protein interaction assays, PCR and qPCR, DNA/RNA extractions, sequencing, cloning techniques, sterile tissue culture, spectrometry, centrifugation, staining and imaging of fixed cells, analysis of complex imaging data (Velocity, ImageJ). Check out more of our research in the Miller Lab here: http://labs.mcdb.lsa.umich.edu/labs/miller/index.php

Member of Dr. Clay Carter’s lab where I investigated hormonal regulation of nectar production and secretion. Uncovered novel regulation of nectar secretion by PIN6, an auxin transport protein. Accomplishments: ● First author publication “PIN6 is required for nectary auxin response and short stamen development” featured on the cover of The Plant Journal in June 2013. ● Presented thesis work at the Biology Departmental Seminar, May 2011. ● Developed novel plant culturing assays that assessed hormonal influence on nectar secretion. On a daily basis I performed various molecular biology techniques including: RT-PCR, qPCR, RNA isolation, DNA isolation, molecular cloning, as well as plant tissue culture techniques of Arabidopsis thaliana and Brassica rapa. Check out more about the research in the Carter lab here: http://www.d.umn.edu/~cjcarter/carterlab.html

I was a teaching assistant for the Biology Department where I taught a Genetics wet lab involving C. elegans genetics and a Molecular Biology wet lab involving molecular cloning of a luciferase gene into E. coli. One a weekly basis I would: ●Prepare pre-lectures for two weekly sessions of Genetics and Molecular Biology Lab. ●Instruct undergraduates in genetic and molecular biology lab procedures and techniques and helped with any issues encountered. ●Teach scientific writing techniques and grade student laboratory reports.

Member of Dr. Ann Kleinschmidt’s lab where I studied differential gene expression of peroxidases in Brassicaceae. Used a combination of RT-PCR, sequencing, and cloning to uncover how a certain class of peroxidase are genetically regulated in model (and nonmodel) plants. Accomplishments: ● Presented poster of senior thesis “Comparisons of sequences and mRNA levels of two class II peroxidases in Arabidopsis thaliana, Brassica rapa, and Alliaria petiolata” at American Society of Plant Biologists (ASPB) meeting in Merida, Mexico, July 2008. ● Secondary author on additional poster presented at 2008 ASPB conference, “Plant Peroxidases: a semester long research project in a molecular biology class” ● Responsible for setting up and establishing protocols for the Biology Department’s newly acquired qPCR machine

Professional certificate course that involves 8 in-depth modules that cover all aspects of global clinical trials. Modules include: -Introduction of ICH-GCP and Historical Framework of Clinical Research -Clinical Study Types, Phases, and Designs -Protocol Development -Informed Consent -Study Activation -Study Conduct -Independent Review Committees in Clinical Trials -Data Collection, Data Management, and Data Quality -Study Close-out and Reporting

Involved six, 5-week courses covering: -Good Clinical Practices -Drug Development Process -Statistical Concepts for Clinical Research -Fundamentals of Site Management -Fundamentals of Clinical Monitoring -Project Management and Leadership in the Healthcare Industry