Maxime Mistretta, PhD.

Spore Biotechnologies is an early stage start-up developing innovative technological tools to help Food&Beverage, Comestics and Pharmaceutics factories manufacture safe products.

Testing co-founding partnerships and ideas with the world’s most ambitious people. Selected from 1000+ applicants to join Entrepreneur First’s platform and work with a community of entrepreneurs and leading advisors to find a co-founder, and build a company from scratch.

I managed and executed three R&D projects: 1. Manage the creation of an analysis pipeline to automatize the treatment of a microscopy image database in Python and based on Machine learning (supervised learning) in collaboration with a computer vision scientist. 2. Lead the valorization of patented technology with a pharmaceutical company (GSK) in a European project with a budget of € 208 million and 31 partners to develop new tuberculosis treatment regimens. 3. Scientifically pilot a microfluidic device's maturation in collaboration with Elveflow to develop an MVP compatible with industrial production to set up a new drug screening service activity inside the Institute Pasteur. In addition, I managed and trained a technician to microfluidics and single-cell analysis to disseminate the technology.

I developed a drug screening platform for long-term single-bacteria tracking adapted to Mycobacterium tuberculosis. I screened for a library of compound to identify molecules to potentiate effect of antibiotic therapy against Tuberculosis. I led the development of an automated pipeline for image analysis in collaboration with two biostatisticians to treat a 60k microscopy image database, reducing the analysis duration by 50-times. I published a patented in 2019 (inventor at 50%) and one article as first author is under reviewing. I presented scientific results in two international conferences (AMR Paris 2018, Nano-biotech Montreux, 2019). In addition, I managed and trained two students (bachelor and master).

We created a prototype for a new miniaturised microfluidic device adapted to time-lapse microscopy imaging of bacteria at a single-cell level to increase the data production throughput by 32x.

MultiPOD Mentoring is a successful and fast growing long-distance, multi-country, professional and personal development program directed to promising young practitioners and future leaders of Global Health and Human Development.

Management & realization of three independent projects: 1. Development of a rapid diagnostic for EBOLA virus in collaboration with Fujifilm: - Antibody screening and selection by ELISA & Bio-Layer Interferometry. - Test supervision in a BSL4 laboratory. 2. Multiplex panel conception based on Luminex xMAP technology . 3. Droplet microfluidics based immunoassay development: - Nanobeads functionalization optimization

The objective of this internship is to develop and integrate a magnetic immunoassay in a droplet-based microfluidic device.

We aimed to quantify the carnitine and acylcarnitines level in Arabidopsis seedlings by HPLC MS/MS. During this trainee period, I worked with Dr. Perrin Yolande. Task performed: - Seed culture in sterile conditions - Lipid extraction - HPLC MS/MS (source ESI)

Project funded by TEKES & Olympus under the leadership of Dr. Hauta-Kasari Markku. Set up of fast and non-destructive tools for forest tree seeds screening using thermal and hyperspectral imaging techniques. The aim was also to study the correlation between metabolomic changes and spectrum signal by TLC and fluorometer.

Two weeks internship in the laboratory of Dr Goffart Steffi. The aims was the study of the duplication and the protection of mitochondrial DNA of mammals organs. Techniques performed: - Mouse cell culture - Production of transgenic bacteria - Mouse ograns DNA extraction - PCR - DNA quantification by spectrometry

Specialty: Conception and Innovation of Bio-products