Matthew Markert

Led upstream development of scalable cell culture processes through commercial readiness, authoring cGMP documentation and providing CMC technical guidance.

Managed lab operations, personnel, testing reports, and documentation for development of assays.

- Developed and optimized CRISPR/Cas9 protocols for both in vivo insect microinjection and in vitro mammalian cell transfection. - Designed, assembled, and validated plasmid constructs for BAC recombination and mammalian chromosomal targeting. - Maintained, passaged, and transfected a variety of mammalian cell lines in support of genetic engineering studies. - Conducted routine viral infection studies in guinea pigs, including blood collection, necropsy, and organ harvesting. - Performed high-throughput PCR genotyping assays to support screening and validation of engineered constructs. - Managed care, breeding, and experimental use of a Monarch butterfly colony. - Parsed, analyzed, and summarized large microarray datasets using advanced Microsoft Office tools to support data-driven conclusions. - Trained, supervised, and mentored undergraduate and graduate student researchers, including rotational students, ensuring adherence to protocols and data quality standards.

- Developed novel techniques for assaying caenorhabditis mating behavior - Collection and maintenance of caenorhabditis breeding lines